GE AKTA avant User Manual page 261

Step
6
Run and evaluate the test
Follow the instructions described in Section 6.1 General performance test actions, on
page 240 to start, run and automatically evaluate the performance test.
Illustration of chromatogram
The illustration below shows a chromatogram from a function test with UV monitor U9-L.
Possible causes of a failed test
The following tables describe possible causes of a failed test. When possible sources of
error have been checked and corrected, repeat the test.
Faulty UV Absorbance Test
Cause
Incorrectly prepared
acetone solution
Wrong UV cell path
length set in UNICORN
ÄKTA avant User Manual 29035184 AE
Action
Prime the buffer inlets and purge System pump A and System pump B. See
Section 5.4.1 Prime buffer inlets and purge system pumps, on page 166.
Action
Make sure that the acetone solution is 1% and that no
solution has evaporated.
See, Calibration of the second UV monitor flow cell length,
on page 331.
6 Performance tests
6.7 UV monitor U9-L test
261