Prepare For A Run At Cold Temperature - GE AKTA avant Operating Instructions Manual

Preparative chromatography system
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5 Prepare the system for a run

5.8 Prepare for a run at cold temperature

5.8

Prepare for a run at cold temperature

Introduction
To fit the ÄKTA avant instrument in a cold cabinet, the foldable door and pump cover
can be removed. For instructions, see ÄKTA avant User Manual. When using the instrument
in a cold room or cold cabinet, make sure to follow the precautions listed in the next
topic.
Precautions concerning runs in
a cold temperature
Note:
Note:
Tip:
128
NOTICE
Avoid condensation. If ÄKTA avant is kept in a cold room, cold
cabinet or similar, keep it switched on in order to avoid conden-
sation.
Avoid overheating. If ÄKTA avant is kept in a cold cabinet and
the cold cabinet is switched off, make sure to switch off ÄKTA
avant and keep the cold cabinet open to avoid overheating.
Place the computer in room temperature. If the ÄKTA avant
instrument is placed in a cold room, use a cold room compat-
ible computer or place the computer outside the cold room
and use the Ethernet cable delivered with the instrument to
connect to the computer.
When the instrument is kept in a cold room, it is important to tighten all tubing
connectors, also the inlet manifold connectors. Otherwise air might get into
the flow path.
Make sure that the instrument, buffers and sample have had time to reach
the ambient temperature. When the instrument has reached the ambient
temperature, calibrate all pressure sensors.
When runs are performed in a cold cabinet, make sure to adjust the target
temperature of the built-in fraction collector temperature control function. The
target temperature is 20°C by default. Settings for the temperature control
function can be edited in the System Settings dialog box of System Control,
or in the Text Instructions pane in Method Editor.
ÄKTA avant Operating Instructions 29101556 AB

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