Before You Prepare The System - GE AKTA avant Operating Instructions Manual

Preparative chromatography system
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5 Prepare the system for a run

5.1 Before you prepare the system

5.1

Before you prepare the system

Introduction
It is important to prepare the system in accordance with the settings in the method to
be run. Before preparing the system, verify the settings in the Method Editor and make
sure that all accessories to be used are available.
Checklist
Prepare the instrument and accessories according to the following information from
the Method Editor:
104
WARNING
Do not use ÄKTA avant if it is not working properly, or if it has
suffered any damage, for example:
-
-
-
Always use appropriate Personal Protective Equipment (PPE)
during operation and maintenance of this product.
Do not use any accessories not supplied or recommended by
GE.
Fire Hazard. Before starting the system, make sure that there
is no leakage.
which valve ports to use for inlets and outlets
which column type to use
which column position to use
which buffers and samples to prepare
which sample application technique to use
make sure that the the pH electrode is connected, if applicable
which cassettes with corresponding deep well plates and/or tubes to use in the
fraction collector, if applicable
if it is a reversed phase chromatography (RPC) run, make sure to follow the safety
precautions below
damage to the power cord or its plug
damage caused by dropping the equipment
damage caused by splashing liquid onto it
ÄKTA avant Operating Instructions 29101556 AB

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