Operation; Procedure Before A Run; Basics Of Performing A Run; Loading The Syringe - GE MicroCal iTC200 Operating Instructions Manual

4 Operation

4.1

Procedure before a run

Note: See Section 1.4 for control software information.
On / Off Instructions
Turning the MicroCal iTC
Once the MicroCal iTC
the cell unit is a power on/off switch, which functions as the master power switch and
must be in the "on" position. It can be turned to "off" when the MicroCal iTC
not be used for long periods of time i.e., weekends, holidays, etc.
Leaving the power on
During frequent "on" periods, the master power may be left as long as the user interface
program, MicroCal iTC
system is does not incur any damage and keeps the MicroCal iTC
Periods of inactivity
GE Healthcare recommends that the MicroCal iTC
master power be turned off, when the system will not be used for extended periods of
time.
4.2

Basics of performing a run

In order to perform a basic ITC titration experiment, the user must load the sample cell
and the syringe, enter the desired parameters into the control software, and click Start.
The reference cell should be filled with water or buffer, and may be left for several days.
4.3

Loading the syringe

To load the titration syringe, place a micro centrifuge tube containing ~100µL of your
sample in the tube holder. Be sure to push the tube to the bottom of the holder with the
MicroCal iTC Operating Instructions 28-9639-77 AA
200
cell on
200
cell has been cabled to the PC, it is ready to use. At the rear of
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NOTICE
The user interface program, iTC200, has to be running for the cell to
function properly even though the power switch is in the "on" position.
, is running. The software automatically ensures that the
200
Procedure before a run 4.1
cell ready.
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application be closed and the
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Operation 4
cell will
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