GE AKTA avant Operating Instructions Manual page 87

Preparative chromatography system
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Step
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ÄKTA avant Operating Instructions 29101556 AB
Action
In the Process Picture:
Click the inlet valve icons. (Click both the Inlet A and Inlet B icons, one
at a time, if both inlets are to be primed.)
Click the position of the inlet to be filled. Fill the positions in reverse al-
phabetical order and start with the highest number. For example, if all
the seven inlets in Inlet Valve B are to be filled, click them in the following
order: B7, B6 . . . B1, assuming that B1 is the starting buffer.
Result: The inlet valve switches to the selected port.
Connect a 25 to 30 ml syringe to the purge valve of one of the pump heads
of System Pump B. Make sure that the syringe fits tightly into the purge
connector.
Open the purge valve by turning it counter-clockwise about three quarters
of a turn. Draw liquid slowly into the syringe until the liquid reaches the
pump.
Close the purge valve by turning it clockwise. Disconnect the syringe and
discard its contents.
Repeat steps 3 to 6 for each piece of inlet tubing that is to be used during
the run. In the final inlet position, draw liquid into the syringe through both
purge valves.
4.5 Prime inlets and purge pump heads
4.5.1 Prime buffer inlets and purge system pumps
4 Installation
87

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