GE AKTA start Maintenance Manual
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GE AKTA start Maintenance Manual

GE AKTA start Maintenance Manual

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Summary of Contents for GE AKTA start

  • Page 1 ÄKTA ™ start Maintenance Manual Original instructions...
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  • Page 3: Table Of Contents

    Table of Contents Table of Contents Introduction ......................About this manual ..........................Important user information ......................Associated documentation ......................Abbreviations ............................System description ....................System overview ........................... Instrument ............................... Maintenance operations ..................Regular maintenance schedule ..................... Clean before planned maintenance or service ..............Access the modules ..........................
  • Page 4 Table of Contents 3.14.4 Switch valve timing ........................3.15 Main board ............................... Troubleshooting ....................UV ................................. Conductivity ............................Frac30 ................................ Pump ................................Pressure sensor ............................. Main board and Power Supply ....................... System related error messages ..................... Troubleshooting flow charts ......................Removing and replacing modules ..............

  • Page 5: Introduction

    1 Introduction Introduction About this chapter This chapter contains important user information and a list of associated documentation. In this chapter Section See page 1.1 About this manual 1.2 Important user information 1.3 Associated documentation 1.4 Abbreviations ÄKTA start Maintenance Manual 29060308 AD...

  • Page 6: About This Manual

    1 Introduction 1.1 About this manual About this manual Purpose of this document The ÄKTA start Maintenance Manual provides you with the instructions needed to unpack, maintain and troubleshoot the ÄKTA start system in a safe way. Nomenclature conventions The nomenclature used in this manual is explained in the table below. Concept Explanation ÄKTA start...

  • Page 7: Important User Information

    1 Introduction 1.2 Important user information Important user information Read this before operating the product All users must read the entire Operating Instructions before installing, operating or maintaining the product. Always keep the Operating Instructions at hand when operating the product. Do not operate the product in any other way than described in the user documentation.
  • Page 8 1 Introduction 1.2 Important user information Safety notices This user documentation contains safety notices (WARNING, CAUTION, and NOTICE) concerning the safe use of the product. See definitions below. WARNING WARNING indicates a hazardous situation which, if not avoided, could result in death or serious injury. It is important not to proceed until all stated conditions are met and clearly understood.

  • Page 9: Associated Documentation

    Introduction This section describes the user documentation that is delivered with the instrument and how to find related literature that can be downloaded or ordered from GE. User documentation on the CD The user documentation listed in the table below is available on the ÄKTA start User Documentation CD.
  • Page 10 1 Introduction 1.3 Associated documentation From the Help menu in UNICORN start or on the UNICORN start DVD, the following user documentation is available. Documentation Main contents UNICORN start User Manual Overview and detailed descriptions of the system control software designed for ÄKTA start, which includes process pic- ture map for real time monitoring, method editor, evaluation and adminis-...
  • Page 11 1 Introduction 1.3 Associated documentation Access documentation from mobile units Scan the code using your mobile phone or tablet computer to access the product page for ÄKTA start. Select documents to download from Related Documents under Product Support. ÄKTA start Maintenance Manual 29060308 AD...

  • Page 12: Abbreviations

    1 Introduction 1.4 Abbreviations Abbreviations Introduction This section explains abbreviations that appear in the user documentation for ÄKTA start. Abbreviations Abbreviation Definition affinity chromatography absorbance unit bitmap file format centipoise (unit of viscosity) column volume demineralized desalting ETFE ethylene tetrafluoroethylene fluorinated ethulene propylene FPGA field-programmable gate array...

  • Page 13: System Description

    2 System description System description About this chapter This chapter provides an overview of ÄKTA start, and the optional Frac30 (Fraction col- lector). For more details, refer to ÄKTA start Operating Instructions. In this chapter This chapter contains the following section: Section See page 2.1 System overview...

  • Page 14: System Overview

    2 System description 2.1 System overview System overview Introduction ÄKTA start is operated and controlled from the Instrument Display. In addition, the UNICORN start software can be used to control ÄKTA start and to analyze the data ac- quired during chromatography runs. UNICORN start offers several additional features that are described in detail in UNICORN start User Manual.
  • Page 15 2 System description 2.1 System overview Illustration of the instrument The illustration below shows the main parts of the instrument. Part Description Function Instrument Display User interface for controlling the system and visualization of the runtime data. Wet side The modules interconnected by tubing have the following functions: to deliver the liquid in a specified flow path •...
  • Page 16 2 System description 2.1 System overview Part Description Function USB port To connect a USB memory stick for storage of results and transfer of files. Note: USB hard drives are not supported. Illustration of the Fraction collector The illustration below shows the Fraction collector (Frac30), showing the front and rear views.
  • Page 17 2 System description 2.1 System overview Part Description Function Power on indicator. Drive sleeve Friction drive to turn the Bowl assembly during fraction collection. Main features of ÄKTA start The main features of ÄKTA start are listed below: • ÄKTA start is a compact and one step purification solution for quick and reliable pu- rification of proteins.

  • Page 18: Instrument

    2 System description 2.2 Instrument Instrument Introduction This section provides an overview of ÄKTA start modules. Illustration of the instrument modules The illustration below shows the locations and gives brief descriptions of the modules placed on the wet side of the instrument. Part Function Description...
  • Page 19 2 System description 2.2 Instrument Part Function Description Sample valve A 3-port valve that allows either the buffer or the sample to enter the flow path. The Sample valve enables direct application of the sample onto the column using the Pump. Pump A peristaltic pump, which delivers buffer or sample to the flow path with a flow rate of up to 5 ml/min.
  • Page 20 2 System description 2.2 Instrument Part Function Description Conductivity The Conductivity Monitor continuously reads the conductivity of the liquid in the Conductivity flow cell. The conductivity is automatically calculated by mul- tiplying the measured conductance by the cell con- stant of the flow cell. The cell constant is factory-calibrated.

  • Page 21: Maintenance Operations

    3 Maintenance operations Maintenance operations About this chapter Regular maintenance of ÄKTA start is essential for reliable function and performance. This chapter provides instructions for periodic maintenance, including calibration and configuration, as well as other required maintenance. In this chapter This chapter contains the following sections: Section See page...
  • Page 22 GE. • Only spare parts and accessories that are approved or supplied by GE may be used for maintaining or servicing ÄKTA start. • Disconnect power. Always switch off power to the instrument before replacing any component on the instrument or cleaning the instrument, unless stated otherwise in the user documen- tation.

  • Page 23: Regular Maintenance Schedule

    3 Maintenance operations 3.1 Regular maintenance schedule Regular maintenance schedule Introduction Regular maintenance should be performed on a daily, weekly and monthly basis. For cleaning instructions, refer to ÄKTA start Operating Instructions. Daily maintenance The following maintenance operations should be performed daily when the system is in use.
  • Page 24 3 Maintenance operations 3.1 Regular maintenance schedule Weekly maintenance The following maintenance operations should be performed weekly or when required. Maintenance action See section Calibrate the Pump. Section 3.6.1 Calibration, on page 50 Visually inspect the inlet filters and clean them ÄKTA start Operating Instructions if necessary.
  • Page 25 3 Maintenance operations 3.1 Regular maintenance schedule Maintenance action See section Calibrate the UV flow cell Section 3.5.1 UV LED calibration, on page 39 Calibrate the Conductivity cell Section 3.11 Conductivity Monitor, on page 68 Pressure sensor zero offset Section 3.12 Pressure sensor , on page 75 Replace the inlet filters ÄKTA start Operating Instructions...

  • Page 26: Clean Before Planned Maintenance Or Service

    Health & Safety Declaration Form for Product Return or Servicing, depending on whether the instrument is going to be serviced on site or returned to GE for service, re- spectively. Copy the form you need from Section 6.1 Health and Safety Declaration Form, on page 155 or print it from the PDF file available on the User Documentation CD.

  • Page 27: Access The Modules

    3 Maintenance operations 3.3 Access the modules Access the modules Instrument modules The location of modules is shown in Illustration of the instrument modules, on page 18. Software access Maintenance and service of the different modules is mamaged from the Instrument Display.
  • Page 28 3 Maintenance operations 3.3 Access the modules Step Action Tap Next or Back to move through the screens 1 to 3. Tap the intended module button to select a specific module for maintenance. ÄKTA start Maintenance Manual 29060308 AD...

  • Page 29: Instrument Display

    3 Maintenance operations 3.4 Instrument Display Instrument Display Introduction This section describes how to calibrate and test the Instrument display. In this section This section contains the following subsections: Section See page 3.4.1 Touch screen calibration 3.4.2 Color test 3.4.3 Diagnostics 3.4.4 Log book Instructions Follow the instructions below to select an option to calibrate and/or diagnose the Instru-...
  • Page 30 3 Maintenance operations 3.4 Instrument Display Step Action Tap Display to access the Instrument Display options. Result: The Display screen opens. ÄKTA start Maintenance Manual 29060308 AD...

  • Page 31: Touch Screen Calibration

    3 Maintenance operations 3.4 Instrument Display 3.4.1 Touch screen calibration 3.4.1 Touch screen calibration Instructions Follow the instructions below to calibrate the Touch screen. Step Action In the Display screen, tap Touch screen calibration. Result: A confirmation screen opens. Tap Yes to proceed with the calibration of the Touch screen. Result: The following screen opens.
  • Page 32 3 Maintenance operations 3.4 Instrument Display 3.4.1 Touch screen calibration Step Action Tap precisely on the marked circle 1. Result: The following screen opens. Tap precisely on the marked circle 2. Result: The following screen opens. Tap precisely on the marked circle 3. Result: The following screen opens.
  • Page 33 Step Action Tap precisely on the marked circle 4. Result: The following screen opens. Tap Exit. Note: If the calibration fails, repeat the test. If the calibration fails again, contact a GE Service Engineer. ÄKTA start Maintenance Manual 29060308 AD...

  • Page 34: Color Test

    Follow the instructions below to test the colors of the Touch screen. Step Action In the Display screen, tap Color test. Result: The following screen opens. Tap Exit. Result: The Color test is completed. Note: If the test fails, contact a GE Service Engineer. ÄKTA start Maintenance Manual 29060308 AD...

  • Page 35: Diagnostics

    3 Maintenance operations 3.4 Instrument Display 3.4.3 Diagnostics 3.4.3 Diagnostics Instructions Follow the instructions below to perform diagnostics of the Touch screen. Step Action In the Display screen, tap Diagnostics. Result: The following screen opens. In the Select backlight brightness screen, tap a radio button to select the intended brightness (%).

  • Page 36: Log Book

    The Log Book displays the number of hours the Instrument Display has been used. The number of hours should be reset to zero when the Instrument Display is replaced. Note: The instrument display must be replaced by a GE Service Engineer. Instructions Follow the instructions below to read the Log book for the Display.
  • Page 37 3 Maintenance operations 3.4 Instrument Display 3.4.4 Log book Step Action If the Instrument Display has been replaced, tap Reset to set the number of hours to 0. Result: A confirmation screen opens. Tap Yes to confirm the reset if the Instrument Display has been replaced. If the Instrument Display has not been replaced, tap No to cancel the action.

  • Page 38: Uv Monitor

    3 Maintenance operations 3.5 UV Monitor UV Monitor Introduction This section describes how to access the UV Monitor options, perform calibrations and edit UV settings. In this section This section contains the following subsections: Section See page 3.5.1 UV LED calibration 3.5.2 Diagnostics 3.5.3 Flow cell path length 3.5.4 Reset number of run hours...

  • Page 39: Uv Led Calibration

    3 Maintenance operations 3.5 UV Monitor 3.5.1 UV LED calibration 3.5.1 UV LED calibration Description UV LED calibration is used for calibrating the UV LED intensity to get the desired response level of the photo detector. Parameter Description Light Light intensity needed to get the expected response at the photo detector.
  • Page 40 3 Maintenance operations 3.5 UV Monitor 3.5.1 UV LED calibration Step Action Tap Yes to automatically adjust the Light strength value to get a minimum Signal response of 2700 mV. Tap Save. Result: The calibrated Light strength value is saved and stored in the perma- nent memory on main board.

  • Page 41: Diagnostics

    3 Maintenance operations 3.5 UV Monitor 3.5.2 Diagnostics 3.5.2 Diagnostics Description Diagnostics is used to conduct a Dark current test and/or a Stray light test to assess electrical noise or presence of stray light. Test Description Dark current test Switches off the UV LED during the test. •...
  • Page 42 3 Maintenance operations 3.5 UV Monitor 3.5.2 Diagnostics Step Action Tap Diagnostics. Result: The Diagnostics screen opens. Tap Set baseline to capture a new reference value for the test. Tap Dark current test. The result is displayed as Pass or Fail: Pass: the UV Monitor is working as it should.
  • Page 43 Make sure that the detector is not exposed to e.g., direct sunlight. If the test fails again, replace the flow cell and then possibly the UV Monitor, or contact a GE Service Engineer. Flush the system with demineralized water so that the Abs value returns close to zero.

  • Page 44: Flow Cell Path Length

    UV LED drift compensation constant. C amb Ambient temperature compensation constant. NOTICE Do not change the C drft and C amb values. These parameters must be set by a GE Service Engineer. Prerequisites • Buffer A: immerse Buffer A inlet into demineralized water. •...
  • Page 45 3 Maintenance operations 3.5 UV Monitor 3.5.3 Flow cell path length Instructions Follow the instructions below to perform a Flow cell path length test and set a new cell length. Step Action Flush the UV flow cell with demineralized water using the Pump, and then leave the UV flow cell filled with water.
  • Page 46 3 Maintenance operations 3.5 UV Monitor 3.5.3 Flow cell path length Step Action Update the calculated Cell length value by using up/down buttons or enter the value. Tap Save path length to save the value to permanent memory. Note: The Abs value should be 340 ± 5% mAU, confirming that normalization has been done.

  • Page 47: Reset Number Of Run Hours

    Tap Configuration. Result: The Configuration screen opens. Do not change the C drft and C amb values. These parameters must be set by a GE Service Engineer. Tap Reset. Result: A confirmation screen opens. If the UV Monitor has been replaced, tap Yes.

  • Page 48: Pump

    3 Maintenance operations 3.6 Pump Pump Introduction This section describes how to perform pump diagnostics and calibration and manage the pump tubing log. In this section This section contains the following sub-sections: Section See page 3.6.1 Calibration 3.6.2 Diagnostics 3.6.3 Pump tubing log Access the Pump service options Follow the instruction below to access the options for Pump calibration and troubleshoot- ing.
  • Page 49 3 Maintenance operations 3.6 Pump Step Action Tap to select the intended option. ÄKTA start Maintenance Manual 29060308 AD...

  • Page 50: Calibration

    3 Maintenance operations 3.6 Pump 3.6.1 Calibration 3.6.1 Calibration Parameter description Parameter Description Flow Rate The intended flow rate used for Pump calibration. Collected Volume The volume of liquid collected for a certain period of time at the set flow rate. The Collected Volume value and the expected value of the volume corresponding to the set flow rate are used internally for calibration.
  • Page 51 3 Maintenance operations 3.6 Pump 3.6.1 Calibration Step Action Use the up/down arrows to set the desired Flow Rate. Tap Start flow to start the Pump. • Collect water for at least one minute in the pre-weighed collection tube. • Tap Stop flow to turn the Pump OFF.

  • Page 52: Diagnostics

    3 Maintenance operations 3.6 Pump 3.6.2 Diagnostics 3.6.2 Diagnostics Parameter description Parameter Description Flow rate Setting of the desired flow rate (ml/min). Pump run Displays the actual number of run hours for the Pump. Check the Pump flow rate Follow the instructions below to perform Pump diagnostics. Step Action Immerse the buffer inlet tubing A in demineralized water.
  • Page 53 3 Maintenance operations 3.6 Pump 3.6.2 Diagnostics Step Action Make sure the flow path and outlet tubing are filled with DM water. • Tap Start flow to run the Pump. • Place the outlet tubing from the Wash valve (Waste port) in a pre- •...

  • Page 54: Pump Tubing Log

    3 Maintenance operations 3.6 Pump 3.6.3 Pump tubing log 3.6.3 Pump tubing log Parameter description Parameter Description Tubing run Indicates how many hours the pump tubing has been • used. When the pump tubing is replaced, use the Reset but- •...
  • Page 55 3 Maintenance operations 3.6 Pump 3.6.3 Pump tubing log Step Action Tap Yes if the pump tubing has been replaced. If the pump tubing has not been replaced, tap No to cancel the action. ÄKTA start Maintenance Manual 29060308 AD...

  • Page 56: Buffer Valve

    3 Maintenance operations 3.7 Buffer valve Buffer valve Description The Buffer valve can be switched to allow the inlet of either buffer A or buffer B, or a mixture of A and B (gradient). Parameter Description Valve switches Indicates how many times the valve has switched be- •...
  • Page 57 3 Maintenance operations 3.7 Buffer valve Step Action In the Settings and service screen, tap Next to access the 2 screen. Result: The following screen opens. In the Settings and service screen, tap Buffer valve. Result: The Buffer valve screen opens. Check that the valve position Buffer A is selected.
  • Page 58 3 Maintenance operations 3.7 Buffer valve Step Action If these checks indicate that the valve is faulty, replace the Buffer valve. If the valve has been replaced, tap Reset to set the Valve switches counter to 0. Result: A confirmation screen opens. Tap Yes to confirm the reset if the valve has been replaced.

  • Page 59: Sample Valve

    3 Maintenance operations 3.8 Sample valve Sample valve Description The Sample valve can be switched to allow the inlet of either buffer or sample. Parameter Description Valve switches Indicates how many times the valve has switched. • When a valve has been replaced, the number of counts •...
  • Page 60 3 Maintenance operations 3.8 Sample valve Step Action In the Settings and service screen, tap Sample valve. Result: The Sample valve screen opens. Check that the Valve position Buffer is selected. • Tap Turn valve to check that the Sample valve switches between Buffer •...
  • Page 61 3 Maintenance operations 3.8 Sample valve Step Action If the valve has been replaced, tap Reset to set the Valve switches counter to 0. Result: A confirmation screen opens. Tap Yes to confirm the reset if the valve has been replaced. If the valve has not been replaced, tap No to cancel the action.

  • Page 62: Wash Valve

    3 Maintenance operations 3.9 Wash valve Wash valve Description The Wash valve can be switched to divert the flow either to column or to waste. Parameter Description Valve switches Indicates how many times the valve has switched. • When a valve has been replaced, the number of counts •...
  • Page 63 3 Maintenance operations 3.9 Wash valve Step Action In the Settings and service screen, tap Next to access the 2 screen. Result: The following screen opens. In the Settings and service screen, tap Wash valve. Result: The Wash valve screen opens. Check that the valve position Waste is selected.
  • Page 64 3 Maintenance operations 3.9 Wash valve Step Action If the valve has been replaced, tap Reset to set the Valve switches counter to 0. Result: A confirmation screen opens. Tap Yes to confirm the reset if the valve has been replaced. If the valve has not been replaced, tap No to cancel the action.

  • Page 65: Outlet Valve

    3 Maintenance operations 3.10 Outlet valve 3.10 Outlet valve Description The Outlet valve can be switched to divert the flow, either to waste or to the Fraction collector. Parameter Description Valve switches Indicates how many times the valve has switched. •...
  • Page 66 3 Maintenance operations 3.10 Outlet valve Step Action In the Settings and service screen, tap Next to access the 2 screen. Result: The following screen opens. In the Settings and service screen, tap Outlet valve. Result: The Outlet valve screen opens. Check that the valve position Waste is selected.
  • Page 67 3 Maintenance operations 3.10 Outlet valve Step Action If these checks indicate that the valve is faulty, replace the Outlet valve. If the valve has been replaced, tap Reset to set the Valve switches counter to 0. Result: A confirmation screen opens. Tap Yes to confirm the reset if the valve has been replaced.

  • Page 68: Conductivity Monitor

    3 Maintenance operations 3.11 Conductivity Monitor 3.11 Conductivity Monitor Introduction This section describes how to perform Conductivity Monitor calibration and edit the Cell constant settings. In this section This section contains the following sub-sections: Section See page 3.11.1 Temperature sensor calibration 3.11.2 Sine generator calibration 3.11.3 Conductivity flow cell calibration 3.11.4 Set reference temperature...

  • Page 69: Temperature Sensor Calibration

    3 Maintenance operations 3.11 Conductivity Monitor 3.11.1 Temperature sensor calibration 3.11.1 Temperature sensor calibration Instructions Follow the instructions below to calibrate the temperature sensor. Step Action Place a precision thermometer in the flow cell path directly after the Con- ductivity flow cell (i.e., immediately after the conductivity tubing which is connected to Outlet valve), and then pump demineralized water through the system with a flow rate of 0.5 ml/min.

  • Page 70: Sine Generator Calibration

    Follow the instructions below to calibrate the sine generator. This must be done when- ever the Main Board is replaced. Note: The Main Board may only be replaced by a GE service engineer. Step Action Use Manual Run to empty any liquid present in the conductivity flow path.

  • Page 71: Conductivity Flow Cell Calibration

    3 Maintenance operations 3.11 Conductivity Monitor 3.11.3 Conductivity flow cell calibration 3.11.3 Conductivity flow cell calibration Prerequisites Calibration solution: • 1.00 M NaCl • 100 mS/cm conductivity standard solution Instructions Follow the instructions below to calibrate the Conductivity flow cell. Note: Temperature compensation is enabled by default (factory setting).
  • Page 72 3 Maintenance operations 3.11 Conductivity Monitor 3.11.3 Conductivity flow cell calibration Step Action Note the current temperature of the calibration solution in the Conductivity flow cell as displayed in the Cell Temperature field. This value is required for setting the reference temperature (see Section 3.11.4 Set reference tem- perature, on page 74).
  • Page 73 3 Maintenance operations 3.11 Conductivity Monitor 3.11.3 Conductivity flow cell calibration ÄKTA start Maintenance Manual 29060308 AD...

  • Page 74: Set Reference Temperature

    3 Maintenance operations 3.11 Conductivity Monitor 3.11.4 Set reference temperature 3.11.4 Set reference temperature Description For the system to calculate the conductivity correctly at different temperatures, the fol- lowing conditions must be met: • Enable Temperature Compensation must be checked in the Calibration screen (see Section 3.11.3 Conductivity flow cell calibration, on page 71).

  • Page 75: Pressure Sensor

    Follow the instructions below to set the pressure to 0 when the Pressure sensor is ex- posed to atmospheric pressure only. NOTICE Calibration of Pressure sensor must be performed by a GE Service Engineer, and must be done when the Pressure sensor has been replaced.
  • Page 76 In the Settings and service screen, tap Pressure sensor. Result: The Pressure sensor screen opens. Note: Stop flow and Calibrate are for use by GE service engineers only. In the Pressure sensor screen, tap Zero offset. Result: A Message screen opens.

  • Page 77: Frac30

    3 Maintenance operations 3.13 Frac30 3.13 Frac30 Introduction This section describes how to enable or disable Frac30, perform diagnostics and handle the run log. In this section This section contains the following subsections: Section See page 3.13.1 Enable or disable Frac30 3.13.2 Diagnostics 3.13.3 Run Log ÄKTA start Maintenance Manual 29060308 AD...

  • Page 78: Enable Or Disable Frac30

    3 Maintenance operations 3.13 Frac30 3.13.1 Enable or disable Frac30 3.13.1 Enable or disable Frac30 Instructions Follow the instructions below to enable or disable Frac30. Step Action In the Settings and service screen, tap Fraction collector. Result: If the Fraction collector is enabled, the following Fraction collector screen opens.

  • Page 79: Diagnostics

    3 Maintenance operations 3.13 Frac30 3.13.2 Diagnostics 3.13.2 Diagnostics Parameter description Parameter Description Feed tube test Checks that Frac30 rotates the Bowl assembly correctly and shifts one tube at a time. Home test Checks that Frac30 rotates the Bowl assembly correctly and shifts from the current position to the home position (tube number 1).
  • Page 80 3 Maintenance operations 3.13 Frac30 3.13.2 Diagnostics Step Action Tap Home test and verify that Frac30 rotates to home position (tube no. 1) from current position. Note: If Frac30 does not rotate the Bowl assembly, check if the Frac30 cable is connected properly to ÄKTA start.

  • Page 81: Run Log

    3 Maintenance operations 3.13 Frac30 3.13.3 Run Log 3.13.3 Run Log Parameter description Parameter Description Run log Displays the number of hours of drive sleeve usage. Instructions Follow the instructions below to reset the Run log. Step Action In the Fraction collector screen, tap Run log. Result: The Run log screen opens.
  • Page 82 3 Maintenance operations 3.13 Frac30 3.13.3 Run Log Step Action If the drive sleeve has been replaced, tap Reset to set the Run time to 0. Result: A confirmation screen opens. Tap Yes to confirm the reset if the drive sleeve has been replaced. If the drive sleeve has not been replaced, tap No to cancel the action.

  • Page 83: System

    3 Maintenance operations 3.14 System 3.14 System Introduction This section describes how to handle firmware updates and export system reports. It also describes how to set delay volumes and switch valve timing. In this section This section contains the following subsections: Section See page 3.14.1 Firmware update...
  • Page 84 4 error messages with Error codes, to a USB memory stick. The latest Firmware ver- sion and FPGA version can also be exported. The data is used by a GE Service Engineer when troubleshooting the instrument. Instructions Follow the instructions below to manage the system options.

  • Page 85: Firmware Update

    3 Maintenance operations 3.14 System 3.14.1 Firmware update 3.14.1 Firmware update NOTICE Before tapping the Firmware update ensure that the system is connected to a stable source of power such as a UPS. During firmware update the system should not be switched off. Prerequisites •...
  • Page 86 3 Maintenance operations 3.14 System 3.14.1 Firmware update Step Action Tap Microprocessor firmware update. Result: The following screen opens. Tap Yes and then wait for approximately 3 minutes for the update to be done. Note: When the firmware update is completed, the instrument automatically restarts and displays the version of the firmware.
  • Page 87 3 Maintenance operations 3.14 System 3.14.1 Firmware update Step Action Once the firmware is updated, perform the following calibration sequence: Display:Touch screen calibration • Pressure sensor:Zero offset • Pump : Calibration:Flow rate • UV:UV LED calibration • Conductivity:Advanced calibration (temperature sensor calibration) •...

  • Page 88: Export System Report To Usb

    Export system report to USB Instructions Follow the instructions below to export the system report to a USB memory stick. Use the system report in further contacts with GE Service Engineers. Step Action Plug the USB memory stick into the USB port located on ÄKTA start.

  • Page 89: Delay Volume Setting

    3 Maintenance operations 3.14 System 3.14.3 Delay volume setting 3.14.3 Delay volume setting Instructions Follow the instructions below to set the delay volume. Step Action In the System screen, tap Delay volume setting. Result: The following screen opens. Enter the internal diameter (ID) and length of the tubing from the Outlet valve to Frac30 in the respective fields, and then tap Save.

  • Page 90: Switch Valve Timing

    3 Maintenance operations 3.14 System 3.14.4 Switch valve timing 3.14.4 Switch valve timing Instructions The switch valve timing may need to be changed if gradients show fluctuations. Follow the instructions below to set the Switch valve timing. For more details, refer to ÄKTA start Operating Instructions section Switch valve timing.
  • Page 91 3 Maintenance operations 3.14 System 3.14.4 Switch valve timing Step Action Tap the radio button to select the required timing: Switch valve timing A (switch time 4 s) • Switch valve timing B (switch time 5 s) • Tap Save to save the timing. Perform Gradient run, either by performing System performance method or manually set the B concentration (Buffer valve) to 50%.

  • Page 92: Main Board

    NOTICE The Main board screen is reserved for operations performed by a GE Service Engineer only. Do not perform any further operations from this screen. Tap Back or Home to return to the previous screen or to go to the Home screen.

  • Page 93: Troubleshooting

    4 Troubleshooting Troubleshooting About this chapter This chapter contains information regarding troubleshooting procedures. Warning messages and Error codes are provided, and possible causes and solutions to those codes are described. In this chapter This chapter contains the following sections: Section See page 4.1 UV 4.2 Conductivity...
  • Page 94 4 Troubleshooting 4.1 UV Warning messages Warning code Description Possible cause Action UV intensity In the Settings and service:UV Flush the UV flow cell and • screen: mount it securely When trying to calibrate; if the detector output is less than 2700 Replace the UV flow cell and •...
  • Page 95 4 Troubleshooting 4.1 UV Warning code Description Possible cause Action Flush UV flow In the Settings and service:UV Flush the UV flow cell and • cell and screen: mount securely. mount secure- When trying to calibrate, if there Recalibrate. • are repeated calibrations and the signal strength is decreasing.

  • Page 96: Conductivity

    4 Troubleshooting 4.2 Conductivity Conductivity Error messages Error code Description Possible cause Action Conductivity Loose cable connector. • module failure Troubleshooting Flow chart 6, on The temperature sensor is • page 107 not functioning. ÄKTA start Maintenance Manual 29060308 AD...

  • Page 97: Frac30

    4 Troubleshooting 4.3 Frac30 Frac30 Error messages Error code Description Possible cause Action Fraction collec- The cable connected be- • tor failure Troubleshooting Flow chart 4, on tween ÄKTA start and Frac30 page 105 is not working. Drive sleeve worn out. •...

  • Page 98: Pump

    Pump Error messages Error code Description Possible cause Action Pump failure Pump is not working. Contact a GE Service Engi- • neer. • Troubleshooting Flow chart 5, on page 106 No flow from The rollers are not rotating. Check the condition of the •...

  • Page 99: Pressure Sensor

    Check the specification and • pressure of the columns for correct flow rate. Pressure sen- The sensor is not connected or Perform Zero offset. • sor failure not calibrated. Contact a GE Service Engi- • neer. ÄKTA start Maintenance Manual 29060308 AD...

  • Page 100: Main Board And Power Supply

    Error messages Error code Description Possible cause Action EPROM error Contact a GE Service Engi- • neer. MPWAtemper- Instrument temperature is out Contact a GE Service Engi- • ature of range (4°C to 35°C) neer.

  • Page 101: System Related Error Messages

    4 Troubleshooting 4.7 System related error messages System related error messages Error messages Error code Description Possible cause Action Method Error. Incomplete method. Reload the method. Reload Wrong method loaded. Method Illegal opcode Incomplete method. Reload the method. Reload Wrong method loaded. Method Illegal Opera- Wrong operations in the system.

  • Page 102: Troubleshooting Flow Charts

    1. Switch the instrument Off. 2. Disassemble the UV Monitor. 3. Replace the UV Monitor and Flow cell. 4. Switch instrument On. Error message still Perform UV LED calibration displayed? Contact GE Service Engineer. Proceed with your work. ÄKTA start Maintenance Manual 29060308 AD...
  • Page 103 1. Switch instrument Off. 2. Disassemble the UV Monitor. 3. Replace the UV Monitor and Flow cell. 4. Switch instrument On. Error message Perform UV LED still displayed? calibration Contact GE Service Engineer. Proceed with your work. ÄKTA start Maintenance Manual 29060308 AD...
  • Page 104 2. Disassemble the UV Monitor. 3. Check that the UV Monitor RJ45 cable is secure; replug properly. 4. Switch on the instrument. Error message Contact GE Service Engineer. still displayed? Proceed with your work. ÄKTA start Maintenance Manual 29060308 AD...
  • Page 105 Replace the Drive sleeve. (Check) Run Frac30 diagnostics. Perform indexing of homing to check if Motor works properly. Does the Motor Contact GE Service Engineer. work properly? Error message Contact GE Service Engineer. still displayed? Proceed with your work. ÄKTA start Maintenance Manual 29060308 AD...
  • Page 106 4 Troubleshooting 4.8 Troubleshooting flow charts Troubleshooting Flow chart 5 ERROR 401 Pump driver Failure Contact GE Service Engineer ÄKTA start Maintenance Manual 29060308 AD...
  • Page 107 Switch on the instrument. 1. Replace the Conductivity Monitor. 2. Enter the new Cell Constant value. Error message still 3. Perform Conductivity calibration. displayed? Has calibration Proceed with your work. passed? Contact GE Service Engineer. ÄKTA start Maintenance Manual 29060308 AD...

  • Page 108: Removing And Replacing Modules

    5 Removing and replacing modules Removing and replacing modules About this chapter This chapter contains instructions how to remove and replace ÄKTA start modules. In this chapter This chapter contains the following sections: Section See page 5.1 3-Port valves 5.2 Mixer 5.3 UV 5.4 UV flow cell 5.5 Pump...
  • Page 109 NOTICE Replacement of modules located on the inside of the instrument must be performed by a GE Service Engineer only. If an internal part needs to be replaced, please contact a GE Service Engineer. Internal modules include: •...

  • Page 110: 3-Port Valves

    5 Removing and replacing modules 5.1 3-Port valves 3-Port valves Introduction This section provides instructions for removal and replacement of 3-port valves, and applies to Buffer valve, Sample valve, Wash valve, and Outlet valve. Illustrations show the Wash valve: the principles are the same for all four 3-port valves. Note that the ori- entation of the valve ports on the instrument is different for the different valves.
  • Page 111 5 Removing and replacing modules 5.1 3-Port valves Instructions Follow the instructions below to remove and replace the valve. WARNING Disconnect power. Always switch off power to the instrument be- fore replacing any component on the instrument or cleaning the instrument, unless stated otherwise in the user documentation.
  • Page 112 5 Removing and replacing modules 5.1 3-Port valves Step Action Slowly remove the valve until a cable connector is accessible. Disconnect the connector. Remove the valve from the instrument. ÄKTA start Maintenance Manual 29060308 AD...
  • Page 113 5 Removing and replacing modules 5.1 3-Port valves Step Action Replace with a new valve. Connect the cable, and then insert the valve gently into the instrument. NOTICE Only replace the valve with the same part number (see Notice, on page 110). Make sure that the ports are aligned to the markings on the instrument chassi, then tighten the retaining screw on the valve fitting.

  • Page 114: Mixer

    5 Removing and replacing modules 5.2 Mixer Mixer Required tools Tool Dimension Torx screwdriver Instructions Follow the instructions below to remove and replace the Mixer. WARNING Disconnect power. Always switch off power to the instrument be- fore replacing any component on the instrument or cleaning the instrument, unless stated otherwise in the user documentation.
  • Page 115 5 Removing and replacing modules 5.2 Mixer Step Action Remove the screw from the Mixer using a T20 screwdriver, supplied with the equipment at delivery. Remove the Mixer. Replace with a new Mixer and reconnect the tubing to the ports. ÄKTA start Maintenance Manual 29060308 AD...
  • Page 116 5 Removing and replacing modules 5.2 Mixer Step Action Switch on the instrument by pressing the Power Switch to the l position. ÄKTA start Maintenance Manual 29060308 AD...
  • Page 117 5 Removing and replacing modules 5.3 UV Required tools Tool Dimension Torx screwdriver Instructions Follow the instructions below to remove and replace the UV Monitor. WARNING Disconnect power. Always switch off power to the instrument be- fore replacing any component on the instrument or cleaning the instrument, unless stated otherwise in the user documentation.
  • Page 118 5 Removing and replacing modules 5.3 UV Step Action Remove the single screw at the top of the UV Monitor, and then the two screws at the bottom using a T20 screwdriver, supplied with the equipment at delivery. ÄKTA start Maintenance Manual 29060308 AD...
  • Page 119 5 Removing and replacing modules 5.3 UV Step Action Gently remove the UV Monitor until the RJ45 connector on the module is accessible. Press the latching tab and disconnect the RJ45 connector from the UV Monitor side. Note: Make sure that the RJ45 connector is not moved inside the cabinet. The con- nector needs to be retained outside the cabinet for re-assembling of a new UV module.
  • Page 120 5 Removing and replacing modules 5.3 UV Step Action Remove the UV Monitor and leave the RJ45 connector as it is. Connect the RJ45 connector to the new UV monitor. Note: Make sure that there is a click sound when the RJ45 connector is connected. The UV monitor may not work properly if the RJ45 connector is not properly connected.
  • Page 121 5 Removing and replacing modules 5.3 UV Step Action Reconnect the tubing to the ports. Switch on the instrument by pressing the Power Switch to the l position. ÄKTA start Maintenance Manual 29060308 AD...

  • Page 122: Uv Flow Cell

    5 Removing and replacing modules 5.4 UV flow cell UV flow cell Instructions Follow the instructions below to remove and replace the UV flow cell. Step Action Disconnect the inlet and outlet tubing from the UV Monitor. Rotate the locknut in the anticlockwise direction. Pull up the UV flow cell.
  • Page 123 5 Removing and replacing modules 5.4 UV flow cell Step Action Place the protective cover around the flow cell to protect the electronics in- side the optical unit from liquid spillage. Note: The protective cover should be assembled after completing the assembly of the UV flow cell inside the UV Monitor by just press-fit.

  • Page 124: Pump

    5 Removing and replacing modules 5.5 Pump Pump Required tools Tool Dimension Torx driver Instructions Follow the instructions below to remove and replace the Pump. WARNING Disconnect power. Always switch off power to the instrument be- fore replacing any component on the instrument or cleaning the instrument, unless stated otherwise in the user documentation.
  • Page 125 5 Removing and replacing modules 5.5 Pump Step Action Open the top cover to remove the tubing from the Pump. ÄKTA start Maintenance Manual 29060308 AD...
  • Page 126 5 Removing and replacing modules 5.5 Pump Step Action Remove the two screws from the Pump using a T20 screwdriver, supplied with the equipment at delivery. Note: Make sure that the pump connector is not moved inside the cabinet. The connector needs to be retained outside the cabinet for re-assembling of a new Pump module.
  • Page 127 5 Removing and replacing modules 5.5 Pump Step Action Gently remove the Pump until the cable connector is accessible. Disconnect the connector. ÄKTA start Maintenance Manual 29060308 AD...
  • Page 128 5 Removing and replacing modules 5.5 Pump Step Action Remove the Pump. Note: After removing the Pump, make sure that the pump cable is placed clear of the fan, on the left side of the instrument. This is to ensure that the cable does not get stuck in the ventilation fan.

  • Page 129: Pump Tubing

    5 Removing and replacing modules 5.6 Pump tubing Pump tubing Instructions Follow the instructions below to replace the pump tubing. WARNING Disconnect power. Always switch off power to the instrument be- fore replacing any component on the instrument or cleaning the instrument, unless stated otherwise in the user documentation.
  • Page 130 5 Removing and replacing modules 5.6 Pump tubing Step Action Place the new tubing between the rollers and the track, press against the pump head inner wall. Note: Make sure that the pump tubing is not twisted or stretched against the rollers. Lower the top cover until it clicks into its fully closed position.
  • Page 131 5 Removing and replacing modules 5.6 Pump tubing Step Action Connect the pump tubing to the Sample valve and to the Pressure sensor. Switch on the instrument by pressing the Power Switch to the l position. ÄKTA start Maintenance Manual 29060308 AD...

  • Page 132: Conductivity Monitor

    5 Removing and replacing modules 5.7 Conductivity Monitor Conductivity Monitor Required tools Tool Dimension Torx screwdriver Instructions Follow the instructions below to remove and replace the Conductivity Monitor. WARNING Disconnect power. Always switch off power to the instrument be- fore replacing any component on the instrument or cleaning the instrument, unless stated otherwise in the user documentation.
  • Page 133 5 Removing and replacing modules 5.7 Conductivity Monitor Step Action Loosen the screws from the Conductivity Monitor using a T20 screwdriver, supplied with the equipment at delivery. Slowly remove the Conductivity Monitor until a cable is visible. The cable is assembled with two connectors interconnected.
  • Page 134 5 Removing and replacing modules 5.7 Conductivity Monitor Step Action Disconnect the connector from the Conductivity Monitor side. Remove the Conductivity Monitor and leave the connector as it is. Note: Make sure that the connector is not moved inside the cabinet. The connector needs to be retained outside the cabinet for re-assembling of a new Conduc- tivity module.
  • Page 135 5 Removing and replacing modules 5.7 Conductivity Monitor Step Action Replace with a new Conductivity Monitor and reconnect the tubing to the ports. Switch on the instrument by pressing the Power Switch to the l position. In the Settings and service screen, access the Conductivity screen to set the cell constant for the new Conductivity flow cell.

  • Page 136: Injection Valve

    5 Removing and replacing modules 5.8 Injection valve Injection valve Introduction Replace the complete valve when internal valve parts are damaged, worn or blocked with salt deposits. Use the Injection valve kit (see Section 5.9 Injection valve kit, on page 139) to service the valve when the external parts and liquid distribution parts are damaged, worn or blocked with salt deposits.
  • Page 137 5 Removing and replacing modules 5.8 Injection valve Step Action Remove the inlet and outlet tubing from the ports. Loosen the screw from the Injection valve using a T10 torx driver, supplied with the equipment at delivery. Remove the Injection valve. ÄKTA start Maintenance Manual 29060308 AD...
  • Page 138 5 Removing and replacing modules 5.8 Injection valve Step Action Replace with a new Injection valve and reconnect the tubing to the ports. Make sure that the ports are aligned to the markings on the instrument chassi. Switch on the instrument by pressing the Power Switch to the l position. ÄKTA start Maintenance Manual 29060308 AD...

  • Page 139: Injection Valve Kit

    5 Removing and replacing modules 5.9 Injection valve kit Injection valve kit Introduction Use the Injection valve kit to service the valve when the external parts and liquid distri- bution parts are damaged, worn or blocked with salt deposits. Replace the complete valve (see Section 5.8 Injection valve, on page 136) when internal valve parts are damaged, worn or blocked with salt deposits.
  • Page 140 5 Removing and replacing modules 5.9 Injection valve kit Step Action Remove the 3 screws on the front side, using the supplied torx driver. Loosen each screw equally in turn, so the distribution plate comes off in parallel to the valve body. Slide the screws out.
  • Page 141 5 Removing and replacing modules 5.9 Injection valve kit Step Action Remove the old channel plate and insert a new one. Remount a new distribution plate so that the marks on the plate match the marks on ÄKTA start. Using the torx driver, tighten the 3 screws in turn, a little at a time, until the distribution plate is fixed to the valve body.

  • Page 142: Frac30 Bowl Assembly

    5 Removing and replacing modules 5.10 Frac30 Bowl assembly 5.10 Frac30 Bowl assembly Remove the Bowl assembly Follow the instructions below to remove the Frac30 Bowl assembly. Step Action Gently move the Dispenser arm counterclockwise to the non-dispensing (end) position. Push the drive assembly laterally and hold it at the retracted position.
  • Page 143 5 Removing and replacing modules 5.10 Frac30 Bowl assembly Step Action Lift and remove the Bowl assembly. Mount the Bowl assembly onto the Bowl holder Follow the instructions below to mount the Frac30 Bowl assembly onto the Bowl holder. Step Action Make sure that the Dispenser arm is in the non-dispensing position.
  • Page 144 5 Removing and replacing modules 5.10 Frac30 Bowl assembly Step Action Note the position of the aligning groove on the Bowl holder. Note the position of the aligning ribs on the Bowl. ÄKTA start Maintenance Manual 29060308 AD...
  • Page 145 5 Removing and replacing modules 5.10 Frac30 Bowl assembly Step Action Hold the Bowl assembly and orient the Bowl with the aligning ribs oriented towards the aligning groove on the Bowl holder. Note: Do not lift the fraction collector by holding the Dispenser arm. Tip: If it is hard to locate the alignment feature in corresponding parts, hold the Bowl assembly near the top of the Bowl holder and rotate the Bowl holder...
  • Page 146 5 Removing and replacing modules 5.10 Frac30 Bowl assembly Step Action Hold the Drive assembly at the retracted position to completely assemble the Bowl assembly on the Bowl holder. ÄKTA start Maintenance Manual 29060308 AD...
  • Page 147 5 Removing and replacing modules 5.10 Frac30 Bowl assembly Remove and replace the drive sleeve Follow the instructions below to remove and replace the drive sleeve. Step Action Cut the old drive sleeve with a sharp knife, and then remove the drive sleeve. ÄKTA start Maintenance Manual 29060308 AD...
  • Page 148 5 Removing and replacing modules 5.10 Frac30 Bowl assembly Step Action Mount a new drive sleeve by pressing and sliding it onto the drive. Remove and replace the Tubing holder Follow the instructions below to remove and replace the Tubing holder. Step Action Pull out the Tubing holder from the Dispenser arm.
  • Page 149 5 Removing and replacing modules 5.10 Frac30 Bowl assembly Step Action Press down the new Tubing holder into the Dispenser arm until it is stopped by the dispensing arm stopping feature. ÄKTA start Maintenance Manual 29060308 AD...

  • Page 150: Fuse

    For continued protection from fire hazard, replace only with the same type and rating of fuse. CAUTION Do not replace the mains fuse if you suspect that there may be a malfunction in the instrument. Contact your GE service representa- tive for advice. Required tools Tool...
  • Page 151 5 Removing and replacing modules 5.11 Fuse Remove the fuse Follow the instructions below to remove the fuse. Step Action Use the flat screwdriver provided, and push the Snap feature on the left side of the fuse holder, in the direction indicated by the arrow mark. Use the flat screwdriver, and push the Snap feature on the right side of the fuse holder, in the direction indicated by the arrow mark.
  • Page 152 5 Removing and replacing modules 5.11 Fuse Step Action Pull the fuse holder out of the mains connector panel by hand. Remove the fuse from the fuse holder. Mount the fuse Follow the instructions below to replace the fuse. Step Action Fit a new fuse of the same type and rating in the fuse holder.
  • Page 153 5 Removing and replacing modules 5.11 Fuse Step Action Align the fuse holder to the rectangular slot in the mains connector panel. Push the fuse holder into the rectangular slot until it fits into the rectangular groove of the mains connector panel. ÄKTA start Maintenance Manual 29060308 AD...

  • Page 154: Reference Information

    6 Reference information Reference information About this chapter This chapter includes Health and Declaration forms for service, and ordering information for ÄKTA start. In this chapter Section See page 6.1 Health and Safety Declaration Form 6.2 Ordering information ÄKTA start Maintenance Manual 29060308 AD...

  • Page 155: Health And Safety Declaration Form

    Service Ticket #: To make the mutual protection and safety of GE service personnel and our customers, all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts a repair. To avoid delays in the servicing of your equipment, please complete this checklist and present it to the Service Engineer upon arrival.
  • Page 156 To make sure the mutual protection and safety of GE personnel, our customers, transportation personnel and our environment, all equipment must be clean and free of any hazardous contaminants before shipping to GE. To avoid delays in the processing of your equipment, please complete this checklist and include it with your return.

  • Page 157: Ordering Information

    6 Reference information 6.2 Ordering information Ordering information For ordering information visit www.gelifesciences.com/AKTAstart. Accessories list Part Accessory description Code no. Pump Marprene Tubing 29024012 Peristaltic Pump 29023992 Solenoid valve Buffer valve 29023895 Sample valve 29023896 Wash valve 29023897 Outlet valve 29023898 Manual Injection valve Injection valve, Manual...
  • Page 158 6 Reference information 6.2 Ordering information Part Accessory description Code no. Fittings Tubing Connector 1/8" 18112117 Ferrule for 1/8" tubing 18112118 Union Luer Female/HPLC Male 18111251 Fingertight Connector 1/16" 18111255 Stop plug 1/16", PKG/5 18111252 Stop plug, 5/16", PKG/5 18111250 Union, 1/16"...
  • Page 159 6 Reference information 6.2 Ordering information Part Accessory description Code no. Miscellaneous Inlet filter assembly 18111315 Inlet filter set, 10 Filters/Nets 18111442 Screw lid GL45 kit, ÄKTA 11000410 Tubing cutter 18111246 Column clamp o.d. 10 to 21 mm 28956319 Short column holder 18111317 T-Slot holders 29024038...
  • Page 160 6 Reference information 6.2 Ordering information Service tools Item Code no. Torx driver T10 29003171 Torx driver T20 28951303 Flat screwdriver 56465600 ÄKTA start Maintenance Manual 29060308 AD...

  • Page 161: Index

    Index Index remove and replace kit, 139 Instrument Display Abbreviations, 12 calibration and tests, 29 ÄKTA start Color test, 34 instrument main parts, 15 diagnostics, 35 instrument modules, descrip- display Log book, 36 tions, 18 Touch screen calibration, 31 intended use, 14 main features, 17 system description, 14 Main board...
  • Page 162 Index Pump, 98 system errors, 101 Safety notices, 8 UV monitor, 94 Safety precautions Typographical conventions, 6 personal protection, 22 Sample valve functional checks, 59 Service and settings flow cell path length, 44 maintenance schedule, 23 flow cell path length test, 45 System reset the run hours, 47 error messages, 101...
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  • Page 164 All goods and services are sold subject to the terms and conditions of sale of Björkgatan 30 the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare repre- 751 84 Uppsala sentative for the most current information.

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