Sample Treatment - Hitachi CHROMASTER 5160 Instruction Manual

3.7.4 Sample Treatment

(a) Example of sample filtration
Filtration with 0.45 μm
membrane filter of cartridge
type
Clean sample
after filtration
(b) Example of inclusion removal from sample
Cartridge of
silica gel
Clean sample after
removal of inclusion
(1) Be sure to filtrate a sample which contains fine-particle
dusts.
Otherwise, the column might be clogged and deteriorated
earlier than normal.
(2) Be sure to remove inclusions from a sample.
Otherwise, data reliability may be degraded due to
appearance of abnormal peaks, etc.
As shown at left, sip a sample into a syringe and
attach a 0.45 μm cartridge-type membrane filter to
the syringe, followed by filtration. Dust in the
sample will be removed efficiently by this method.
NOTE:
In analysis with a reversed-phase column (such
as ODS column), for example, take a sample into
a syringe and filtrate it with a normal-phase
cartridge attached as shown at left. By this
method, inclusions can be removed.
NOTE:
3 - 63
There are various sample filtration
methods.
Select a method suitable for the
volume of your sample.
There are various methods of
removing inclusions from a sample.
Select the optimum removal method
according to your analytical purpose.
3.7.4