Sample Pretreatment - Hitachi CHROMASTER 5160 Instruction Manual

3.5.2 Sample Pretreatment

condition as salt precipitation or development of algae or
microbes.
To prevent this condition, be sure to carry out the above
procedure before leaving the autosampler unused for a
long time.
While the autosampler is stopped, air will dissolve into the
residing solution through the Teflon material of the
connection tubing. Therefore, if a long-time stop is made
on a flow path section, the solution in the connection tubing
will be in an insufficient degassed state. At the time of
restarting the autosampler, displace the solution in the
connection tubing with a new one thoroughly, and then
proceed to analytical operation.
(1) Filter samples without fail if they may contain fine dust.
Dust can clog column and deteriorate it.
(2) Remove foreign matter from samples without fail.
Foreign matter can lower the reliability of data, e.g.
generating abnormal peaks etc.
(a) Example of Filtering Samples]
Dust in sample can be removed easily as follows.
Take sample into a syringe, attach cartridge type
0.45 μm membrane filter, and perform filtering.
See the figure at left.
NOTE: There are various ways of filtering sample.
3 - 72
Filter the sample in the way suitable for the
sample amount.
Filtering by cartridge
type 0.45 μm
membrane filter.
Filtered clean sample