Hitachi CHROMASTER 5160 Instruction Manual page 637

1.2 Configuration of Liquid Chromatograph System
1.2.2 Low-Pressure Gradient System 1.2.2 Low-Pressure Gradient System
(1) An eluent is fed by the pump through the degassing unit.
(2) A sample is injected from the autosampler.
(3) The injected sample is separated through the column which
is maintained at a constant temperature by the column oven.
(4) Each component separated from the sample is then
detected as a signal by the detector.
In the low-pressure gradient system, two or more solvents are In the low-pressure gradient system, two or more solvents are
mixed in its low-pressure section from which a liquid feed is mixed in its low-pressure section from which a liquid feed is
performed by a single pump. The composition of an eluent is performed by a single pump. The composition of an eluent is
made to vary with time in chromatographic separation. made to vary with time in chromatographic separation.
Injection valve
Syringe valve
Degassing
unit
Fig. 1-2 Low-Pressure Gradient System Fig. 1-2 Low-Pressure Gradient System
1 - 4
Wash fluid
Mobile phase
Chromaster
organizer box
Chromaster 5410/
5420/5430 detector
Chromaster 5310
column oven
Chromaster 5210
autosampler
Washing pump
Chromaster 5110 pump
Drain
Low-pressure gradient unit
proportioning valve