Hitachi CHROMASTER 5160 Instruction Manual page 906

(b) Two types of wash fluids are applied to the
autosampler. Select a wash fluid according to Table
3-3 for Isocratic analysis, and Table 3-4 for Gradient
analysis.
(c) When using one wash fluid that fills the washing
requirements, the wash fluid1 side must be used.
(Can't use the wash fluid2 only) In this case, when an
aqueous solvent is applied to the wash fluid2 side, it
can be used as a plunger seal washing of the mobile
phase deliver pump.
(d) As a wash fulid in cases where a solvent containing a
buffer solution or salt has been fed as the mobile
phase, use a mobile-phase solvent containing no salt.
(For example 20 mmol / L KH
is used as a mobile phase, H2O:CH3CN ＝ 50:50 is
applied)
If a solvent containing salt is used for washing, there
will occur salt precipitation inside the syringe to
damage its glass surface or tip, resulting in
degradation in performance or shorter service life.
(e) Avoid using an alkaline solvent (pH 8 or higher) for
washing, as it may deteriorate the glass surface of the
inside of the syringe, resulting in degradation in
performance or shorter service life.
(f) When the all loop injection method is applied, a wash
fluid in a buffer tube and a sample are introduced to
the flow path together. And it may cause degradation
in a peak shape of a chromatogram and reproducibility.
In this case, select two types of wash fluid according to
Table 3-3 and 3-4.
NOTE: When unsuitable reagents absolutely necessary for
ingredient separation or reaction of a constituent have
been used, or when mobile phase which deposits salt
has been used, wash all the flow paths with distilled
water after completing analysis without fail. If not, the
plunger may wear down. Especially, pour water into
the rear part of pump head to wash it out. Plunger
Cleaning Mechanism (option) is separately prepared.
It is recommended to use this option.
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PO : CH CN ＝ 50:50
2 4 3