Analyzing Operation - Hitachi CHROMASTER 5160 Instruction Manual

3.2.2 Analyzing Operation

(1) Routine analysis operation
Make sure the pump, column, detector, data processing unit, etc.
are correctly connected, and then set the mobile phase and
detergent. Check that the waste bottle is not filled with waste
solution.
Start the pump and wait until the flow path is filled with the mobile
phase.
Turn on power supply to the autosampler. When initialization has
been normally terminated, the idle status is set and the monitor
screen appears.
STEP RACK VIAL VOL(µL) INJ TIME [I]
1 1 1 10.0 1/1 0.0 [C]
Wait until the pump, column, detector, etc. are warmed up and the
baseline becomes stable.
Press the WASH key to fill the syringe and wash port with
detergent.
Place the vials containing samples in the rack and position the rack
in the autosampler.
Press the START key to start a sequential program.
After completion of the sequential program, the monitor screen
reappears. When ALL END is set, ALL END will be displayed after
the set ALL END time.
NO
End of analysis
YES
Turn off power supply.
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