Hitachi CHROMASTER 5160 Instruction Manual page 796

(1) Each of plural mobile phases is fed from the degassing unit
to the low-pressure unit.
(2) In the low-pressure unit, the mobile phases are mixed at a
pre-specified ratio.
(3) Through the mixer, the mixture mobile phase is delivered.
(4) A sample is injected by the autosampler.
(5) The injected sample is separated through the column which
is maintained at a constant temperature by the column oven.
(6) Each component separated from the sample is detected as
a signal by the detector.
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