Preparation Of Operation - Hitachi CHROMASTER 5160 Instruction Manual

4.1.2 Preparation of Operation

(3) Performance Check Parts Attached to Chromaster 5210
Autosampler
• Standard sample
60% methanol solution of methylparaben
(Methylparaben 6 mg / Methanol 50 mL / Water 40 mL)
• Sample vial, cap, septum
• Standard rack
NOTE: Performance specification should be checked in
the following combination. Avoid using the non-
slit septum or the sealed plastic cap to acquire
original good reproducibility performance.
Part No.
080-3595
855-2351
080-3704
In the other cases (refer to 6.3), adjust the rack
parameters according to the combination used for
your practical application (refer to 3.2), and check
the reproducibility performance before proceeding
to operation.
NOTE: For good reproducibility, avoid using a septum
repeatedly to prevent a cause of a hole. It causes
a fluctuation of sample concentration in sample
vial.
NOTE: Be sure to use the sample vials, caps and septa
recommended by Hitachi High-Technologies (refer
to 6.3) If other parts are used, the instrument
may malfunction or its original performance may
not be available.
(1) Figure 4-1 shows how to connect the units for performance
check. According to this figure, carry out tubing.
Before energizing the system, ensure that the tubing
between the autosampler and pump/detector is properly
connected.
4 - 2
Part Name
1.5 mL Sample vial
Septum (Si -TEF), slit (-)
Screw cap