Colorimetric Imaging - GE Amersham ImageQuant 800 User Manual

3 Handle methods in ImageQuant 800 control software
3.5 Colorimetric image capture

3.5.1 Colorimetric imaging

3.5.1 Colorimetric imaging
Introduction
Colorimetric imaging is used to image colorimetric stained gels (e.g., Coomassie and
silver staining). This setting can also be used for quantitative densitometry measurements.
The table below states which accessories are needed for colorimetric imaging. This de-
pends on whether Epi or Trans illumination is being used. Epi illumination can be per-
formed on the upper or lower tray position. Trans illumination can be performed in the
lower tray position only.
Imaging mode
Epi illumination
Trans illumination
Note:
The Colorimetric tab
The illustration below shows the main components of the Colorimetric screen.
Part
1
62
Black tray with White insert
Glass tray
To achieve the best possible accuracy, trans illumination is recommended.
This method is only available in certain instrument configurations. For more
information on instrument configurations and upgrading the Amersham
ImageQuant 800 instrument, see Amersham ImageQuant 800 Operating
Instructions.
Function
Save image capture settings as
a method
Part Function
4 Open method
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