Optical Densitometry - GE Amersham ImageQuant 800 User Manual

3.5.3

Optical Densitometry

Introduction
For optical density (OD) measurements, pixel data are converted to OD values to obtain
a linear correlation between the output signal and the amount of sample. Each instrument
is factory calibrated using an OD step tablet. In this mode, basic settings are predeter-
mined and cannot be changed.
Note:
Take an OD image
To take an OD colorimetric image, use the following steps.
Step
1
2
3
4
5
Amersham ImageQuant 800 User Manual 29383813 AA
3 Handle methods in ImageQuant 800 control software
OD measurements can only be made in the lower tray position using Trans il-
lumination.
Action
On the Home screen, tap Colorimetric image capture.
Under 1.Select Exposure, tap OD measurement.
Under Advanced settings, change the settings for Capture area as required.
See Section 3.3 Advanced settings, on page 41
To save the capture settings for future use, tap Save method. In the popup
window, enter the Name of the method and tap Save.
Tap Start.
Note:
Both color and greyscale images for analysis are saved in the image file
folder.
3.5 Colorimetric image capture
3.5.3 Optical Densitometry
for more information.
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