Introduction - GE Amersham ImageQuant 800 User Manual

3.1

Introduction

Amersham ImageQuant 800
control software methods
A method is the combination of all settings that affect an image capture. This includes
imaging mode, exposure, tray position, and any advanced setting such as binning or
image area. Methods can be saved and opened at a later time. If an opened method is
changed, it needs to be saved again. Image files contain information on the method and
image settings used. See help text in the Amersham ImageQuant 800 control software
for more information.
Available imaging modes
The Amersham ImageQuant 800 supports three modes of image capture: chemilumines-
cence, colorimetric, and fluorescence. The main functions and uses of these modes are
described in the table below.
Image capture mode
Chemiluminescence
Colorimetric
Fluorescence
Amersham ImageQuant 800 User Manual 29383813 AA
3 Handle methods in ImageQuant 800 control software
Imaging options
Chemiluminescence
With colorimetric marker
Gel documentation
OD measurement
Fluorescence
With colorimetric marker

3.1 Introduction

Sample type
Enzymatically labelled
membranes (e.g.,
horseradish peroxidase)
Colorimetric markers (e.g.,
Amersham Rainbow™
Markers)
Colorimetric stained gels
(e.g., Coomassie and silver
staining)
Quantitative densitometry
measurement
Fluorescent labelled
membranes,
DNA gels (e.g., ethidium
bromide and alternatives
staining)
Colorimetric markers (e.g.,
Amersham Rainbow
Markers)
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