Time Series Chemiluminescence Exposure - GE Amersham ImageQuant 800 User Manual

3.4.4

Time series chemiluminescence exposure

Introduction
A time series exposure takes a series of sequential images.
Time series settings
The Time series exposure setting has two additional settings that can be altered. These
are described in the table below
Setting
Cumulative
Stop when saturated
Take a time series exposure
To take a time series chemiluminescence exposure, use the following steps.
Step
1
2
3
4
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Description
This setting will sum the pixel intensity of all of the previously
captured images. For example, the second image will be
the sum of the intensity of the first and second image. The
third image will be the sum of the first, second, and third
images. This setting can be used to determine the optimum
exposure time for the sample.
Image exposure will stop as soon as regions start becoming
saturated. In a time series exposure, cumulative imaging
will also stop when saturation has been reached. This means
that subsequent images will not be taken.
Action
On the Home screen, tap Chemiluminescence image capture.
Under 1.Select Exposure, tap Time series.
Under Number of images, use the + and - buttons to select the desired
number of image to be taken.
Under Exposure time per image, tap the white box.
3.4 Chemiluminescence image capture
3.4.4 Time series chemiluminescence exposure
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