Fluorescence Imaging - GE Amersham ImageQuant 800 User Manual

3.6.1

Fluorescence imaging

Introduction
Fluorescence imaging is used to image fluorescent samples, for example in gels, mem-
branes, multi-well plates, and petri dishes. For membranes with colorimetric markers,
such as Amersham Rainbow markers, additional colorimetric image capture produces
an overlay image comprising color and fluorescence image captures.
Note:
The Fluorescence tab
The illustration below shows the main components of the Fluorescence screen.
Part
1
2
3
4
5
Amersham ImageQuant 800 User Manual 29383813 AA
3 Handle methods in ImageQuant 800 control software
For best image quality in Fluorescence imaging mode, it is recommended to
not use the White insert due to auto-fluorescence from the insert. If a colori-
metric image is needed, for example from a gel with Colorimetric marker, it is
recommend to capture images firstly with the White insert for Mw determina-
tion and then without the White insert for best fluorescence detection of weak
bands.
Function
Save image capture settings as
a method
SNOW (noise reduction mode)
Manual exposure
Auto exposure
Open method
3.6 Fluorescence image capture
3.6.1 Fluorescence imaging
Part Function
8 Select binning
9 Select dyes
10 Select capture area
11 Toggle colorimetric marker
12 Toggle full dynamic range
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