Colorimetric Capture - GE Amersham Imager 680 Operating Instructions Manual

5.5.3

Colorimetric capture

Introduction
Colorimetric capture is available in Amersham Imager 680 configurations as described
in the following table.
Option
Epi-
illumination
Trans-
illumination
Both options use white light imaging sources. For Epi-illumination the light source is lo-
cated at the top of the Amersham Imager 680 interior. For Trans-illumination the sample
is illuminated from the light source at the bottom of the instrument interior. When using
Trans-illumination, the intensity data is converted to optical density (OD values), which
provides a more quantitative result compared to Epi-illumination.
Note:
The images are by default created with automatic exposure time.
The table below describes details on accessories and tray positions for colorimetric image
capturing.
Option
Epi-
illumination
Amersham Imager 680 Operating Instructions 29271308 AB
Sample
Membrane
•
Gel
•
Gel
•
Colorimetric capture is only available with Lower tray position.
Tray and accessory
Black
Tray +
White In-
sert
White
Trans
Tray
Illumination 680
Epi-White x
Trans-White
5 Operation
5.5 Capture an image
5.5.3 Colorimetric capture
680 680 680
UV QC RGB
x x x
x x
Tray position
Lower tray position
Lower tray position
137