Automatic Chemiluminescence Exposure - GE Amersham ImageQuant 800 User Manual

3 Handle methods in ImageQuant 800 control software
3.4 Chemiluminescence image capture

3.4.2 Automatic chemiluminescence exposure

3.4.2 Automatic chemiluminescence exposure
Introduction
Automatic exposure uses a short pre-capture exposure to automatically calculate the
exposure time. The region of interest used to calculate the exposure time can be manu-
ally selected using Pre-capture if needed. The calculated exposure time is based on the
intensity level of the most intense signal detected in the selected region, or in the entire
capture area if no region is predefined. The Full Dynamic Range setting can be used to
use the full range of intensities to calculate the exposure time. This results in a longer
exposure time.
Take an automatic exposure
To take an automatic exposure, use the following steps.
Step
1
2
3
4
5
6
50
Action
On the Home screen, tap Chemiluminescence image capture.
Under 1.Select Exposure, tap Auto.
If an overlay image of the sample and marker is required, tap the toggle
button next to Colorimetric marker.
If shorter exposure times are required, tap the toggle button next to Full
dynamic range to the off position.
Note:
Turning off Full dynamic range will reduce the range of intensity levels used
in the image.
Under Advanced settings, change the settings for Binning and Capture
area as required. See
information.
Enter the settings for Fluorescence multiplex if required, see
multiplex, on page 47.
Section 3.3 Advanced settings, on page 41
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Fluorescence