Automatic Fluorescence Exposure - GE Amersham ImageQuant 800 User Manual

3 Handle methods in ImageQuant 800 control software
3.6 Fluorescence image capture

3.6.2 Automatic fluorescence exposure

3.6.2 Automatic fluorescence exposure
Introduction
Automatic exposure uses a short pre-exposure to automatically calculate the exposure
time. The area of interest is used to calculate the exposure time can be manually selected
using Pre-capture. The calculated exposure time is based on the intensity level of the
most intense signal detected in the selected region, or in the entire capture area if no
region is predefined. The Full Dynamic Range setting can be used to use the full range
of intensities to calculate the exposure time. This results in a longer exposure time.
Take an automatic exposure
To take an automatic exposure, use the following steps.
Step
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Action
On the Home screen, tap Fluorescence image capture.
Under 1.Select Exposure, tap Auto.
Under Select dyes, select the dye or dyes being used in the image.
Tap Select to return to the method screen.
If an overlay image of the sample and marker is required, tap the toggle
button next to Colorimetric marker.
If shorter exposure times are required, tap the toggle button next to Full
dynamic range to the off position.
Note:
Turning off Full dynamic range will reduce the range of intensity levels used
in the image.
Amersham ImageQuant 800 User Manual 29383813 AA