Create A Method - GE AKTA avant User Manual

5 Operation
5.9 Create a method and perform a run

5.9.1 Create a method

5.9.1 Create a method
Introduction
The predefined methods are built up using phases, where each phase corresponds to
a step in a chromatography run with a number of properties associated with that phase.
See UNICORN Method Manual for more information about method structure, definitions
and concepts of methods in UNICORN.
Note:
Predefined methods
There are several predefined methods to choose from. All the predefined methods are
listed in the following topics.
The predefined methods available for each system are defined by the Instrument Con-
figuration. See Section 9.6 Predefined methods and phases, on page 449 for more informa-
tion about each method.
Purification methods
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Maintenance methods
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UNICORN methods are always created specifically for a designated system
and thus also for a specific system type. However, it is often useful to convert
a method that was originally created for a system of one type, for use with a
system of another type. For example it is possible to convert a method created
for ÄKTA avant 25 to ÄKTA avant 150. See UNICORN Method Manual.
Affinity Chromatography (AC)
Anion Exchange Chromatography (AIEX)
Cation Exchange Chromatography (CIEX)
Chromatofocusing (CF)
Desalting
Gel filtration (GF)
Hydrophobic Interaction Chromatography (HIC)
Manual Loop Fill
Reversed Phase Chromatography (RPC)
Column CIP
Column Performance Test
Column Preparation
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