GE AKTA avant User Manual page 159

The path length of the UV flow cell might differ from the nominal length, which leads to
inexact results in the calculation of protein concentration in the eluate. To achieve nor-
malized absorbance, the path length of the UV flow cell must be calibrated and the cal-
culated flow cell path length set manually. See Calibration of the second UV monitor flow
cell length, on page 331. This allows UV data from runs made with different UV flow cells
(but with the same nominal path length) to be compared directly.
Prepare the inlet tubing
Connect inlet tubing to the inlet ports that are to be used, and place all inlet tubing that
is to be used during the method run in the correct buffers.
Note:
Prepare the outlet tubing
Connect outlet tubing to the outlet ports of Outlet valve that are to be used. If the external
Fraction collector F9-R is to be used, make sure that the tubing is connected as described
in Connect tubing to the ÄKTA avant instrument, on page 134, and prepare the fraction
collector. Otherwise, place the outlet tubing in suitable tubes or flasks.
Prepare the waste tubing
All waste tubing is found on the rear of the instrument.
ÄKTA avant User Manual 29035184 AE
When using high viscosity buffers or samples in combination with high flow
rates it is recommended to change to tubing with larger inner diameter or
shorten the length of the tubing.
2
1
5 Operation
5.2 Prepare the flow path
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