GE AKTA avant User Manual page 85

Scanning of cassettes
When the fraction collector drawer is closed automatic scanning is performed. There
are two types of scanning procedures:
•
•
Fractionation modes to avoid
spillage
Two fractionation modes are available which avoid spillage between wells or tubes
during fractionation:
•
•
Note:
Note:
Fractionation arm positions
•
•
ÄKTA avant User Manual 29035184 AE
Full scan: Scanning of cassette type codes to identify which cassette types are used,
and scanning of rows and columns in deep well plates to identify which plate types
are used (24, 48, or 96 wells). Full scan is performed only when the system is in state
Ready.
Quick scan: Scanning of cassette type codes to identify which cassette types are
used. Quick scan is performed during the run to ensure that correct cassettes are
placed in the fraction collector.
Accumulator: The accumulator is used to collect liquid during movement between
wells, tubes or bottles. The liquid is then dispensed in the next well or tube. Fraction-
ation with accumulator can be used at all flow rates.
DropSync: When using DropSync the sensors in the dispenser head detect when a
drop is released from the nozzle. The dispenser head moves to the next well or tube
just after a drop is released. Fractionation with DropSync can be used at flow rates
up to 2 ml/min. Solutions with low surface tension may require a lower flow.
DropSync is used in ÄKTA avant 25 only.
If using the accumulator with buffers with high salt concentration, it is recom-
mended to regularly use Accumulator Wash between or in methods.
Home position: The home position is used when the fraction collector is idle. The
fractionation arm is positioned in the front of the interior of the fraction collector
and the dispenser head is positioned over the waste funnel. This position is called
Waste (Frac) in UNICORN.
Frac cleaning position: The fraction collector cleaning position is used for convenient
cleaning of the dispenser head. The fractionation arm is positioned in the front of
the interior of the fractionation collector and the dispenser head is moved to the
center of the fractionation arm.
3 Standard instrument modules
3.10 Built-in fraction collector
3.10.1 Function
85