GE AKTA avant User Manual page 507

Standard delay volumes in ÄKTA
avant 25 and ÄKTA avant 150
The table below shows the delay volumes of different sections of the flow path.
Flow path
UV detector to built-in fraction collector
Note:
With pH electrode and flow restrictor off-line.
UV detector to Outlet Valve
Note:
With pH electrode and flow restrictor off-line.
UV detector to second Outlet Valve
UV detector to third Outlet Valve
Flow restrictor,
including tubing 1R and 2R
pH flow cell
UV detector to Fraction collector F9-R, 2nd
(With pH electrode and flow restrictor off-line.)
Delay volumes for extended
systems
If modules and tubing are added to, or subtracted from, the ÄKTA avant system between
the UV monitor and the fraction collector, use the standard delay volumes values in
Standard delay volumes in ÄKTA avant 25 and ÄKTA avant 150, on page 507 and correct
with tubing volumes from Section 9.3 Tubing and connectors, on page432 and component
volumes from Section 9.13 Component volumes, on page 509 to calculate a new delay
volume. Add this delay volume in UNICORN.
Note:
ÄKTA avant User Manual 29035184 AE
For pH-valve V9-pH and V9H-pH always use the volume for the valve in by-
pass position. The system automatically adds the volumes for the flow restrictor
and the pH flow cell when if they are part of the system.
9 Reference information
9.12 Delay volumes
Volume (μl)
ÄKTA avant 25: 518
ÄKTA avant 150: 1807
ÄKTA avant 25: 142
ÄKTA avant 150: 535
ÄKTA avant 25: 196
ÄKTA avant 150: 781
ÄKTA avant 25: 250
ÄKTA avant 150: 1028
ÄKTA avant 25: 48
ÄKTA avant 150: 94
ÄKTA avant 25: 76
ÄKTA avant 150: 129
ÄKTA avant 25: 240
ÄKTA avant 150: 928
507