GE AKTA avant User Manual page 158

5 Operation
5.2 Prepare the flow path
ÄKTA avant 150
Three different Mixer chambers are delivered with ÄKTA avant 150. Their volumes are:
1.4 ml, 5 ml (mounted at delivery), and 15 ml.
Mixer chamber
volume (ml)
1.4
5
15
Inlet tubing size for ÄKTA avant 150 in BufferPro and quaternary gradients
When running BufferPro or quaternary gradients with ÄKTA avant 150 at flow rates below
10 ml/min, it is possible to optimize performance by changing the pieces of tubing marked
InA and InB from standard tubing dimension i.d. 2.9 mm, o.d. 3/16" to tubing dimension
i.d. 1.6 mm, o.d. 1/8" (delivered with the instrument).
Note:
Select UV flow cells
Flow cells with shorter path lengths are suitable to use for high protein concentrations.
Flow cells with longer path lengths are suitable to use for low protein concentrations.
UV flow cells for UV monitor U9-M
UV flow cells are available with three different path lengths; 0.5 mm, 2 mm (default) and
10 mm.
The real cell path length of the UV cell is automatically recognized by the monitor when
a cell is fitted. The UV data is normalized to the nominal path length. This allows UV data
from runs made with different UV flow cells (but with the same nominal path length) to
be directly compared.
UV flow cells for UV monitor U9-L
UV flow cells for UV monitor U9-L are available with two different path lengths; 2 mm
(default) and 5 mm. When replacing a UV flow cell, the path length must be set in the
System Control module if concentration calculations will be done in the Evaluation
module. Use the nominal flow cell length if the UV flow cell is replaced but not calibrated.
See Update the cell path length, on page 334 to set the flow cell path length.
158
Flow rate (ml/min),
Binary gradient
0.5 to 15
2 to 25
5 to 150
Remember to change back to standard tubing size for higher flow rates.
Flow rate (ml/min),
Quaternary and BufferPro
gradients
N/A
2 to 10
10 to 40
ÄKTA avant User Manual 29035184 AE