GE AKTA avant User Manual page 366

8 Troubleshooting
8.3 Troubleshooting: Monitors
Problem
Ghost peaks
366
Possible cause and action
Air in the UV flow cell
•
Use the Flow restrictor. Use the pH valve instruction
to manually set the Flow restrictor inline (Flow path:pH
valve:Restrictor in-line), or select the Flow restrictor
in the Method Settings phase of a method.
Remove the air by flushing the cell with water or
buffer. If persistent, clean the UV cell. See Section 7.5.1
Clean the UV flow cell, on page 283.
Air in buffers
•
De-gas if necessary.
Dirt in the UV flow cell
•
Clean the UV cell. See Section 7.5.1 Clean the UV flow
cell, on page 283.
Dirt in the flow path
•
Clean the system in accordance to Section 7.6.2 Per-
form System CIP, on page 289. Clean the column in ac-
cordance to Section 7.6.3 Perform Column CIP, on
page 297.
ÄKTA avant User Manual 29035184 AE