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CHAPTER 3
General Technical Considerations
Table 1 Test result with bacillus subtilis (CFU/30L air)
No.
1
2
3
4
5
Average
Table 2 Test result with pseudomonas diminuita (CFU/30L air)
No.
1
2
3
4
5
Average
(7) Considerations
Bacillus subtilis is commonly detected in the air and resistant to dry. Pseudomonas diminuita is susceptible to dry
and only a few exists in the air. However, it is used in the performance verification of the bacteria trapping filter
since the particle size is small (Cell diameter; 0.5 µm: Cell length 1.0 to 4.0 µm).
Both Bacillus subtilis and Pseudomonas diminuta are detected at the location A and B in the outlet side duct where
they are sprayed, but neither them are detected at location C (in the air filtered by the HEPA filter) and the location
D (in the air crossed in the Lossnay Core) on the inlet side.
Since the number of bacteria in the location A is substantially equal to one in the location B, it is estimated that
only a few bacteria are attached to the Lossnay Core on the outlet side. Also, no test bacteria is detected at the
location D where the air is crossed in the Lossnay Core. Therefore, it can be concluded that the bacteria attached
to the outlet side will not pass through the inlet side even after the heat is exchanged.
Shunji Okada
Manager, Biological Section
Kitasato Reseaarch Center of Enviromental Seiences
30
A
4
5.4 × 10
5.6 × 10
3
8.5 × 10
7.5 × 10
3
7.5 × 10
4
1.2 × 10
1.2 × 10
4
1.8 × 10
1.5 × 10
4
2.0 × 10
1.5 × 10
A
5
3.6 × 10
2.9 × 10
5
2.5 × 10
1.2 × 10
5
2.4 × 10
7.2 × 10
5
3.4 × 10
8.4 × 10
5
1.7 × 10
3.8 × 10
5
2.7 × 10
4.7 × 10
B
4
< 10
3
< 10
3
< 10
< 10
4
< 10
3
< 10
4
< 10
B
5
< 10
5
< 10
5
< 10
5
< 10
5
< 10
5
< 10
C
D
3
< 10
3
< 10
3
< 10
3
< 10
3
< 10
3
< 10
C
D
3
< 10
3
< 10
3
< 10
3
< 10
3
< 10
3
< 10
3
3
3
3
3
3
3
3
3
3
3
3
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