GE SE 250 Mighty Small II Operating Instructions Manual page 38

5 Operation
5.2 Assembly
Step
1
2
3
Final assembly
38
Action
To aid in loading samples, wet the well-locating decal and apply it to the
front of the glass plate so that the appropriate edge outlines the sample
wells.
Note:
The side wells for standards of a preparative comb correspond to the outer-
most wells formed by the 10-well comb.
Fill the sample wells and each upper buffer chamber that will be used with
running buffer. One upper buffer chamber holds approximately 75 mL.
Load the sample into the wells using a fine-tipped microsyringe. The width
of the wells depends on the number of wells per comb.For the volume re-
quired to fill to 1mm well depth, see the table below.
Volume of sample (µL) per 1 mm depth
No. of wells Comb thickness (mm)
0.75
5 9.5
9
10 3.6
15 2.2
18
CAUTION
Devices must only be operated with a certified power supply capa-
ble of limiting the voltage and current to specified ratings.
1.0 1.5
12.7 19.1
5.8
4.8 7.2
2.9 4.4
2.9
SE 250/260 Mighty Small II Operating Instructions 29281629 AA