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5 Operation
5.2 Assembly
Clamp the sandwich in place
Note:
Step
1
2
3
4
Sample preparation and loading
If applicable, cast the stacking gel in the unit.
Calculate the stacking gel monomer solution volume: measure the distance, in cm, from
the top of the resolving gel to the notch in the alumina plate. (This should be at least 2
cm—more if the sample depth in the well is unusually high.) Multiply this distance by the
gel width (8.3 cm) and the gel thickness (cm). This product is the required volume in ml.
If wells are already in place, start at with preparing the sample.
36
Cooling is optional. If desired, attach tubing to ports on both sides of the core
before attaching gel sandwiches. Circulate coolant. See
page
32.
Action
Lightly press the sandwich against the gasket and secure it to the core with
one spring clamp on each side.
Position the jaw so that the shorter rounded jaw edge fits into the core
groove and the longer edge sits on the glass plate.
Slide the clamps down to the stop.
Note:
Proper positioning is important to achieve a seal and to minimize glass
breakage.
Repeat step 1-3 for the second sandwich, or, if running only one gel, clamp
a plain glass plate on the unused side of the core to prevent a possible short
circuit with the unused electrode. Do not fill this chamber with buffer if no
gel sandwich is in place.
SE 250/260 Mighty Small II Operating Instructions 29281629 AA
Optional cooling, on
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