GE SE 250 Mighty Small II Operating Instructions Manual page 37

To prepare the wells, follow these steps:
Step
1
2
3
4
5
6
To prepare the sample, follow these steps:
Step
1
2
3
4
To load samples, follow these steps:
SE 250/260 Mighty Small II Operating Instructions 29281629 AA
Action
De-aerate the stacking gel monomer solution.
Add catalyst and initiator to the stacking gel monomer solution and then
pour.
Use a pipette to deliver the solution into one corner of the plate, taking care
not to trap any bubbles.
Insert a comb (at a slight angle to prevent trapping air) into the sandwich,
allowing the comb sides to rest on the spacers.
Overlay each gel with a thin layer of water-saturated n-butanol, water, or
diluted gel buffer to prevent gel exposure to oxygen. Slowly deliver the
overlay solution from a glass syringe fitted with a 22-gauge needle. Apply
the solution near the spacer at the side of the sandwich and allow it to flow
across the surface unaided.
Allow a minimum of one hour for the gel to polymerize.
Action
Increase liquid sample density with 10% glycerol or sucrose.
Add a tracking dye.
For SDS protein gels, use 2× treatment buffer to denature both liquid and
dry samples in a test tube.
To liquid protein solutions, add an equal volume of 2× buffer.
To dry protein samples, add equal volumes of buffer and ddH
the desired concentration.
Heat the tube in boiling water for 90 seconds, then chill it in ice until ready
to use.
Treated samples can be stored at -40°C to -80°C for future runs.
5 Operation
5.2 Assembly
O to achieve
2
37