Before You Prepare The System - GE AKTAavant Getting Started

7.1 Before you prepare the system

Introduction
It is important to prepare the system in accordance with the settings in the method to
be run. Before preparing the system, check the settings in the Method Editor and make
sure that all accessories to be used are available.
Checklist
Make sure to prepare the system in accordance with the settings in the method to be
run. Remember to check:
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Getting Started with ÄKTAavant and UNICORN 6 28-9440-69 AB
which valve ports to use for inlets and outlets
which cassettes with corresponding deep well plates and/or tubes to use in the
Fraction collector, if applicable
which column type to use
which column position to use
which sample application technique to use
that the pH electrode is connected, if applicable
if it is a reversed phase chromatography (RPC) run
WARNING
RPC runs with 100% acetonitrile. Always replace the green
PEEK tubing between the used system pump and the pressure
monitor with orange PEEK tubing, i.d. 0.5 mm, before running
RPC with 100% acetonitrile. Set the System pressure alarm to
10 MPa.
WARNING
Fraction collector. Do not fractionate flammable liquids. When
running RPC methods, or other procedures using solvent based
buffers, collect fractions through the outlet valve.
7 Prepare the system for a run
7.1 Before you prepare the system
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