GE AKTAavant Getting Started page 89

Step
5
6
7
8
9
10
Getting Started with ÄKTAavant and UNICORN 6 28-9440-69 AB
Action
Fill a syringe with approximately 10 ml of the first pH standard buffer.
Connect the syringe to the Luer connector in pH valve port Cal, and inject
the buffer.
When the Current value is stable, click
Wash the pH flow cell by injecting water into pH valve port Cal using a new
syringe.
Enter the pH of the second pH standard buffer in the pH for buffer 2 field.
Repeat steps 5-6 using the second pH standard buffer.
Result: The calibration date and time are displayed in the dialog, and also
values for Calibrated electrode slope and Asymmetry potential at pH 7.
Is the Calibrated electrode slope ≥ 80% and the Asymmetry potential at
pH 7 inside the interval ±60 mV?
•
If Yes: Click
and to close the Calibration dialog.
If No: Clean the pH electrode, and repeat the calibration procedure. If
•
this does not help, replace the electrode. For information about cleaning
and replacing the pH electrode, see User Manual Chapter Maintenance.
CAUTION
pH-electrode. Handle the pH-electrode with care. The glass tip
may break and cause injury.
7 Prepare the system for a run
to switch the pH valve back to the default position,
7.5 Calibrate the pH monitor
.
89