GE AKTAavant Getting Started page 64

6 Create a method
6.1 Guide to method creation
6.1.1 Create and edit methods
Choose a maintenance method
A number of predefined methods for preparation and cleaning are available. Use these
maintenance methods to prepare and clean the system and columns, and to fill the
system and columns with storage solution. The table below gives suggestions for what
methods and solutions to choose for the different purposes.
If you wish to...
Prepare tubing and instrument modules
before a run
Prepare column before a run
Clean tubing and modules
Clean column
Leave system in storage solution
Leave column in storage solution
TIP:
TIP:
Note:
64
CIP (Cleaning-In-Place) and preparation of columns and system may
be run either as separate predefined methods or as phases included
in chromatographic methods.
The System CIP and System Preparation phases are designed to use
one cleaning solution each. The System Preparation method includes
two System Preparation phases and the System CIP method includes
three System CIP phases. To use additional solutions sequentially, add
phases to the method.
Usually 0.5-1 M NaOH is used for cleaning of the system and column.
However, before choosing cleaning agent always consider the media
and buffers used, and also the chemical resistance of the column.
Phase/Method
System Preparation
Column Preparation
System CIP
Column CIP
System CIP
Column CIP
Getting Started with ÄKTAavant and UNICORN 6 28-9440-69 AB
Solution
suitable buffers
suitable buffers
0.5-1 M NaOH
0.5-1 M NaOH
20% Ethanol
20% Ethanol