GE AKTAavant Getting Started page 66

6 Create a method
6.1 Guide to method creation
6.1.2 Predefined methods
Method
Desalting (DS)
Gel filtration (GF)
Hydrophobic
Interaction
Chromatography
(HIC)
Reversed Phase
Chromatography
(RPC)
System CIP
System
Preparation
66
Description
After equilibration and sample application, the proteins are
eluted isocratically. This technique is commonly used for buffer
exchange.
After equilibration and sample application, proteins separate
and elute according to their size (largest first).
After equilibration and sample application (use a buffer
containing a high salt concentration, for example 2 M
Ammonium Sulphate) hydrophobic proteins are adsorbed to
the column ligand. After a wash to remove unbound sample,
elution is performed using a gradient of decreasing salt
concentration. Finally, the column is washed and
re-equilibrated with start buffer.
After equilibration and sample application, hydrophobic
proteins adsorb to the column ligand. After a wash to remove
unbound sample, elution is performed by generating a gradient
of a non-polar, organic solvent such as Acetonitrile. Finally,
the column is washed and re-equilibrated.
The system is filled with cleaning solution. Select for example
inlets, outlets and column positions to be cleaned. Three
System CIP phases are included in the method to facilitate
the use of three different cleaning solution. Additional System
CIP phases can be added from the Phase Library if desired.
The system is filled with preparation solution. Select for
example inlets, outlets and column positions to be prepared.
Two System Preparation phases are included in the method.
Additional System Preparation phases can be added from
the Phase Library if desired.
Getting Started with ÄKTAavant and UNICORN 6 28-9440-69 AB