Apply Sample From A Superloop - GE AKTA pilot 600 User Manual

4 Operation
4.1 Sample application

4.1.3 Apply sample from a Superloop

4.1.3 Apply sample from a Superloop
Introduction
Samples may be applied to the column from a Superloop, connected to the column valve
directly before the column. Superloop 150 SNAP (capacity 150 mL) is fitted with SNAP
connections.
Instructions for handling the Superloop, including filling with sample and cleaning, are
given in the Superloop 150 SNAP Instructions, supplied with the Superloop.
When to use
Use of a Superloop is recommended for:
•
•
Note:
Mounting the Superloop
The Superloop should be mounted close to the column valve to keep connecting tubing
short. Mounting in an ÄKTA accessory holder on a module front panel with rails (see
Module front panel with rails, on page
columns and accessories, on page
Follow the steps below to mount and connect the Superloop.
Step
1
2
3
68
CAUTION
Do not use a Superloop without its protective jacket. The Superloop
may crack if exposed to overpressure.
sample volumes in the range 10 to 150 mL
Size exclusion chromatography and desalting (to minimize band broadening)
Sample application from a Superloop can only be used with downflow through
the column.
Action
Fill the Superloop eluent chamber with buffer and sample chamber with
sample (see the separate Superloop 150 SNAP Instructions)
Mount the Superloop on or close to the ÄKTA pilot 600 instrument, with the
sample chamber downwards.
Connect the Superloop eluent inlet (top connection) using SNAP connectors
to the top port for the unused column position on the column valve.
25) or an Extension Stand (see
24) is recommended.
ÄKTA pilot 600 User Manual 29274559 AA
Extension stand for