Calibration; Placing The Sample - GE ImageQuant LAS 4000 User Manual

4 Operating the ImageQuant LAS 4000
4.2 Preparation for exposure
Step Operation
Wait until the CCD has reached the preset cooling temperature and is ready for use.
3
TIP:

4.3 Calibration

Ensure that the ImageQuant LAS 4000 is properly calibrated. See
page 104

4.4 Placing the sample

This section describes how to choose an appropriate sample tray, and how to place the sample for
exposure.
1 Select a sample tray suitable for the type of exposure to be performed.
Detection
Chemiluminescence
Bioluminescence
34
• The instrument will be ready in a few minutes. The power LED on the instrument is
lit blue when the instrument is ready.
• Method/Tray position and Focusing can be prepared even if the CCD has not cooled
completely.
• The Start button can be clicked even when the CCD is not completely cooled with
the EtBr trans method.
and Section 5.12.2 Flat Frame Calibration, on page
Sample type
Membrane
Titer plate
Section 5.12.1 CCD Calibration, on
105.
Tray
Epi tray
NP tray
ImageQuant LAS 4000 User Manual 28-9607-42 AB