Block 6: Sample Load - GE AKTA ready Operating Instructions Manual

10.2.7

Block 6: Sample load

The block responsible for sample load has the sample volume as finishing criterion. Flow
and valve positions (air trap inline and column inline) are maintained from the previous
block and do not have to be redefined.
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ÄKTA ready Operating Instructions 28960345 AD
Action
Create a block named
Before starting sample injection, you need to auto-zero the UV sensor. This
is done by inserting an AutoZero_UV_AT131 instruction (group Alarms&Mon).
At a volume of 2 liters, set the inlet to that which your sample container is
connected by inserting an Inlet instruction and setting this to, for instance,
Inlet1. Insert also an Outlet instruction set to Outlet1. Set both AirTrap and
Column to ByPass.
Insert an InjectionMark (in group FlowPath) at 2 liters. This will set the time
and volume to zero when the sample inlet valve is opened.
In order to fill the flow path with
sample liquid start a manual flow,
which is stopped when the sample
reaches the UV sensor (i.e., when the
UV readings reach above a defined
limit). The two instructions required
for this are:
ManFlow (in group Pump), which starts the flow and maintains it at a
•
set level, in this case 50% (ManFlow is not controlled by the flow meter).
Hold_Until (in group Watch), which stops the flow when UV_AT131
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readings are Greater_Than a set limit, in this case 200 mAU. Timeout is
set to, for instance, 15 liters.
When the flow kit is filled with sample liquid, the Column is again set to Inline
and a Flow instruction set to, for instance 300 cm/h, is added.
Finally, set the End_Block instruction to equal the volume of your sample,
for example 42.8 liters.
10.2 An example of using the Method Editor
and set its base to Volume.
MySampleLoad
10 Creating a method

10.2.7 Block 6: Sample load

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