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3 Operation
14
Complete filling
In this method a surplus of sample is used to ensure that the sample loop
is filled completely, allowing reproducible sample volumes. In preparative
applications the sample volume should be at least 2 times the volume of
the sample loop. For analytical reproducibility a sample volume 5 times the
volume of the sample loop should be used.
With complete filling the sample volume can only be changed by changing
the loop size.
Complete filling is achieved as follows:
1 Set valve to position LOAD.
2 Load the syringe with sample (2-5 times the loop volume).
3 Gently load the syringe contents into the loop.
4 Leave the syringe in position. The sample will be injected onto the
column when the valve is switched to INJECT in the method.
Note: If the syringe is taken out before the sample is injected onto the
column, self drainage will occur and the loop will be emptied.
Emptying the sample loop
When emptying the sample loop a buffer volume of approx. 5 times the
sample loop volume should be used to flush the loop and ensure that all
sample is injected onto the column.
Storage
Overnight: The valve can be left filled with a buffer.
Weekend or Long time storage: Flush the valve with water and then fill it
with 20% Ethanol.
Valve INV-907 Instructions 56-8101-01 Edition AE
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