GE AKTA ready Operating Instructions Manual page 109

Stage
After:
Failure:
Prepare a test
Wash liquid and test solution are required for a component test. A Test solution prepara-
tion protocol template is available on the system CD. The volumes depend on the flow
kit used:
•
•
Test solution for the High Flow Kit is prepared as follows: 10 liters of 1.0 M NaCl with 1%
acetone in purified water (i.e., 584 g NaCl + 100 ml acetone in 9.90 liters purified water).
For the Low Flow Kit, half the amounts are used.
Note:
Step
1
ÄKTA ready Operating Instructions 28960345 AD
Description
After the test has finished, the test results can be printed. The results printout
shows all registered flow kit bar codes, installation procedures, notes, and
test results. If page 8 does include any warning or other text the test failed.
If all flow cell tests passed the system can be used.
If any part of the test fails, there is a troubleshooting guide available (see
Section 8.3 Troubleshooting a failed component test, on page
ÄKTA ready High Flow Kit — 25 liters of wash liquid and 10 liters of test solution.
ÄKTA ready Low Flow Kit — 10 liters of wash liquid and 5 liters of test solution.
The test solution should be freshly made, so the acetone does not evaporate.
Action
Remove the protective end cap from
inlet 6 (arrow), and connect the wash
liquid.
Note:
Containers must not be placed at a
level lower than 1 m below the inlets
(i.e., floor level).
8 Component test
8.1 Run a component test
117).
109