GE AKTA pilot 600 User Manual page 70

4 Operation
4.1 Sample application
4.1.3 Apply sample from a Superloop
Setting
Loop position
Empty loop with
Use the same inlets as
Method Settings
Fill the system with
selected buffer
Fractionation settings
70
Description
Shows the column valve position used by the Superloop.
This setting can only be changed by changing the Column
position in Method Settings.
The specified volume will be pumped through the Superloop
on to the column. If it is important that all of the sample is
applied to the column, enter a volume approximately 10 mL
greater than the volume of sample in the Superloop.
This option controls the source of the buffer pumped into
the Superloop eluent chamber during sample application.
Select this option to fill the system flow path with the buffer
before applying the sample to the column.
Settings specified here determine how flowthrough during
sample application will be collected. See
tionation, on page 71
Fractionation during sample application is generally rele-
vant only for adsorption chromatography.
Section 4.2 Frac-
for details of fractionation settings.
ÄKTA pilot 600 User Manual 29274559 AA