GE AKTA pilot 600 User Manual page 69

Step
4
The illustration below shows a column mounted as Column 1 and a Superloop mounted
as Column 2. The arrows indicate flow direction.
Part
1
2
Sample Application phase
properties
To apply sample from a Superloop, select Inject sample from Superloop, manual load
in the Phase Properties for the Sample Application phase. Set the parameters as de-
scribed in the table below. For details of settings in the Phase Properties that are not
described here, see the UNICORN online help.
ÄKTA pilot 600 User Manual 29274559 AA
Action
Connect the Superloop sample outlet (bottom connection) using SNAP con-
nectors to the bottom port for the unused column position on the column
valve.
Description
Eluent chamber
Sample chamber
4.1 Sample application
4.1.3 Apply sample from a Superloop
1
2
4 Operation
69