GE AKTA pilot 600 User Manual page 67

Step
2
3
4
5
6
7
ÄKTA pilot 600 User Manual 29274559 AA
Action
Insert the end of the sample inlet tubing into the sample container.
Tip:
If the tubing is filled with buffer, introduce a small air bubble into the sample
tubing between the buffer and the sample. This will mark the progress of the
sample front along the flow path tubing.
Set a suitable pump flow rate, for example 100 mL/min. Follow the sample
front in the tubing. (The sample front may be difficult to follow if the flow
rate is too high.)
When the sample front has passed through the inlet valve, click Pause
Switch the inlet valve to buffer inlet.
Click Continue , change the flow rate to maximum (600 mL/min), and
wait until all air bubbles have been removed through the Waste 2 outlet on
the column valve.
Result: The flow path contains sample up to the inlet valve and buffer from
the inlet valve to the column valve.
Click End .
4.1 Sample application
4.1.2 Apply sample from a system inlet
4 Operation
.
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