ABB Totalflow NGC8206 Startup Manual page 22

NOTE: In the Manual Peak Find screen, changes to Gate Times and Peak
Labeling may be seen immediately by selecting Post Process. All changes
in the pressure or times windows at the right of the screen will be reflected
following a Run Single Cycle.
Gating Peaks
Gate On and Gate Off times in the Peak Setup table in the Manual Peak
Find screen instructs the process when to start and stop looking for peaks.
Each Gate On/Gate Off time applies the parameters in its row to the peaks
in its time frame. The Gate On time should begin in an area prior to the first
component peak and in a relatively flat area on the baseline. Likewise, the
Gate Off time should be on a flat area and not fall during a component
peak.
Make changes in the Peak Setup table in the Peak Find screen. Send
Setup and then Post Process to see updated chromatograms.
Labeling Peaks
If peaks are integrated correctly and column pressures are within range but
no labels display, the user may need to label the peaks. Manually label the
peaks in the Peak Find screen by zooming in on the chrom, placing the
cursor inside of the peak, right clicking and selecting Label Peak. When the
new window displays, select the component for that peak from the drop-
down selection, and click the Label Peak button. Continue until all peaks
are labeled.
Send Setup, and click the Post Process button. Wait for the screen to
update the chromatograms.
Forward Flow Duration
A small peak (part of C6+) displaying after the nC5 peak indicates that the
Forward Flow is too long. It may be necessary to shorten the Forward Flow
Flow/Inject Time. Make small time increment changes to avoid over
compensation. Make adjustments to the Forward Flow Time on the Manual
Peak Find screen, send Setup and run a Single Cycle. Repeat as
necessary. If the Cal Blend component concentrations IC5 and nC5 are
similar, the peak areas should be within 3% of each other. If using
Totalflow's standard blend, IC5 and NC5 are approximately 0.1%.
If water causes a problem, the user may need to increase the
Backflush/Reverse Time. For additional information, see the chapter on
troubleshooting in the NGC8206 User's Manual.
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