Connect A Column - GE AKTApure User Manual

5.5 Connect a column

Introduction
This section describes how to connect a column to the instrument using a column holder and without
introducing air into the flow path. Several types of column holders are available for ÄKTA pure.
Methods automatically include a pressure alarm based on the specifications of the chosen column
type. However, when running manual runs you have to set the pressure limits yourself. Also, to protect
the column media, special settings are needed. See Section 5.6 Pressure alarms, on page 177 for more
information on pressure alarms.
Note:
Note:
Attach a column holder and connect a column
Follow the instructions below to connect a column to the instrument. Always use a column holder. If
a column valve is used, connect the column to the appropriate A and B ports on the valve. If no column
valve is used, connect the column directly to the flow path tubing. Use appropriate tubing and connec-
tors. The instructions below show a system configured with Column valve V9-Cs.
Step
1
ÄKTA pure User Manual 29119969 AB
WARNING
To avoid exposing the column to excessive pressure, make sure that the pressure
limit is set to the specified maximum pressure of the column. Before connecting a
column to the ÄKTA pure instrument, read the instructions for use of the column.
Do not overtighten when connecting columns. Overtightening might rupture the connectors
or squeeze the tubing and thereby result in high back pressure.
If no column valve is used, remove the column from the system before running a system wash.
The pressure during a system wash may become too high for the column.
Action
Attach an appropriate column holder to the rail on the instrument.
5 Operation

5.5 Connect a column

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