Column Preparation; Starting A Run - GE ÄKTA start Manual

3. Tighten all connections and pump DM water through
the system to check for leaks.
4. Tighten any leaking connector.
Removal from cold room
1. Switch off the instrument and disconnect power cable.
2. Loosen all connections to prevent them sticking when
the system returns to room temperature.
3. Allow the instrument to stabilize at room temperature.
4. Tighten all connections and pump DM water through
system to check for leaks.

Column preparation

The Column preparation method is used for:
• Preparing a new column
• Equilibrating the column.
Note: Equilibrate columns before starting a new run.
Instruction
1. Immerse the buffer inlet tubing into the buffer used in
the chromatographic run.
2. Connect the column in the flow path.
Operation from the Instrument Display
Prepare system
Column preparation
Set Run parameters to
suit your requirement
Edit run
Operation from UNICORN start
UNICORN start
System control
System preparation
Column preparation
6
ÄKTA start
Method run
Run End
Exit
Method run
Pre defined

Starting a run

Chromatography runs can be performed either by running
predefined methods, or by using manual operations.
Perform a method run
The following method types are available with ÄKTA start
to perform chromatography runs:
• Quick start
• Templates
• User defined
Follow the workflows below to run predefined methods.
Operation from the Instrument Display
Select method
• Quick start
• Templates
• User defined
Set Run parameters to
suit your requirement
Edit run
Operation from UNICORN start
UNICORN start
System control
Select method
• Quick start
• Templates
• User defined methods
ÄKTA start System Cue Card, 29-0240-42 AC
ÄKTA start
Method run
Select
Run End
Exit
Method run
Pre defined
Ok/Start