Column Pressure And Over-Pressure - GE AxiChrom 50 Operating Instructions Manual

2 Safety instructions
2.1 Safety precautions
2.2

Column pressure and over-pressure

AxiChrom columns have been stringently tested for their pressure tolerances and the
effects of over-pressure. At high over-pressure levels, the borosilicate glass will tend to
snap rather than shatter and splinter when filled with liquid.
Section 3.2 Primary specifications, on page 22 presents the maximum pressure that may
be used for packing and operation procedures using AxiChrom 50, 70 and 100. For safe
use of the columns, these pressure limits shall be observed. Particular attention shall be
taken when connecting manual pumps to the column, or deviation in normal operating
procedures using ÄKTAexplorer or ÄKTApilot that can lead to an over-pressure. AxiChrom
70 and 100 columns are not compatible with ÄKTAexplorer.
GE Healthcare recommends that the pressure inside the column is continually measured
to detect and deal with any potential over-pressure situations. It is also recommended
that over-pressure safety equipments are fitted. For instructions on how to handle
overpressures, see Handling over-pressure in the column, on page 51.
14
NOTICE
Perform a leakage test whenever any of the wetted parts have
been re-fitted or changed.
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG