GE Amersham ImageQuant 800 User Manual page 77

Step
7
8
Amersham ImageQuant 800 User Manual 29383813 AA
3 Handle methods in ImageQuant 800 control software
Action
In the pre-capture screen, tap Select next to Step 1: Select area of interest
(1). Tap and drag to select the area of interest (2).
Tap Select next to Step 2: Select background. Tap and drag to select the
background area.
Note:
The SNOW algorithm calculates signal and noise from the region of interest
and the selected background area. Select a flat background area that is free
from bands, stain speckles, or dust. If it is not possible to select such a back-
ground area, for example if a post-stained gel exhibits many dye speckles in
the gel background, it is recommended to select a smaller area, or an area
next to the gel."
3.6 Fluorescence image capture
3.6.4 SNOW fluorescence exposure
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