Hitachi CHROMASTER 5160 Instruction Manual page 682

3.7 Preparation
Devices to be prepared
(i) Ultrasonic cleaner
(Put water in the bath without fail.)
(ii) Aspirator with rubber stopper which fits the mouth of
mobile phase container
Rubber stopper
should fit the mouth
of container.
Mobile phase container
(a) Set mobile phase container* in ultrasonic cleaner after
sufficient stirring.
* When using a mixed mobile phase, stir it completely
by using a stirrer, or else poor repeatability or drift
of baseline may be caused.
NOTE: Degas mobile phase of aqueous solution by using both
ultrasonic cleaner with water and aspirator to decrease
pressure. Fix the degassing time.
(b) Insert rubber tube of aspirator into mouth of reagent
bottle.
(c) Turn on the power of ultrasonic cleaner.
(d) Open the faucet of city water for aspirator to decrease
pressure.
(e) When degassing, small bubbles are generated first,
then gradually larger bubbles are generated.
Continue degassing until bubble generation finishes.
It takes about 5 minutes. (See NOTE.)
(f) When bubble generation finishes, remove rubber tube
of aspirator from the reagent bottle.
(g) Shut off the city water.
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Ultrasonic cleaner
Aspirator