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BEFORE COMMENCING OPERATION
Connection of N
WARNING
Check the gas type and ensure that it is fit for the purpose.
connected correctly and are not liable to become disconnected. Ensure that the gas pressure is
set at the specified value. Improper connection of the gas pipe or use of incorrect gas pressure
may result in leakage of gas. Elevated level of gas can be hazardous to health and may lead to
asphyxiation and risk of death.
This incubator needs N
When the setting of O
When the setting of O
O
density in the atmosphere is about 20%. For the control of O
2
diluted by N
gas when the setting of O
2
gas is added when the setting of O
1. Install a pressure regulator (optional accessory MCO-100L) on N
regulator rated at 25MPaG (250kgf/cm
30psiG) on the secondary side.
2. Using the gas supply tube provided, connect the pressure regulator to the N
at the rear left side of the incubator.
sub-cylinder to the inlet B so that the gas supply can be switched automatically when one gas cylinder is
empty. Connect the same gas type to both inlet A and B. Never connect N
same time to the inlet A and B respectively.
3. Set the N
or O
2
2
injection). The higher gas pressure makes control range of O
pressure may cause disconnection of internal pipes inside the incubator, which will result in leakage of N
or O
gas into the atmosphere. Elevated level of CO
2
may lead to asphyxiation and risk of death or fire by gas leakage. The repair of the incubator will be
necessary if the internal pipe is disconnected.
4. Check that no gas is leaking at any point where the pipe connects with the gas regulator or the
incubator.
Note:
• Refer to "Procedure for replacement of gas cylinder" enclosed with the unit at the time of replacement.
• The incubator, including the gas supply pipes and services must be examined at frequent intervals to
ensure they are safe. Ensure that items such as pipes are replaced if there is any sign of deterioration.
WARNING
O
gas increases the susceptibility of substances to burn. Take care of the handling of flame in a room
2
where the incubator is installed.
19
(or O
2
or O
gas depending on the setting of O
2
2
density is less than 18%: N
2
density is more than 22%: O
2
density is less than that of the atmosphere. On the contrary, O
2
density is more than that of the atmosphere.
2
2
G, 3600psiG) on the primary side and 0.2MPaG (2.0kgf/cm
Connect the main cylinder to the inlet A and connect the
gas pressure on the secondary side to 0.05MPaG (0.5kgf/cm
) gas cylinder
2
Make sure that all pipes are
density. The selection is as follows:
2
gas
2
gas
2
density in the chamber, O
2
2
density wider. In addition, excessive
2
gas or O
gas can be hazardous to health and
2
2
75
2
or O
gas cylinder. Or use a
2
or O
inlet (A/B) located
2
2
gas and O
gas at the
2
2
2
G, 7.1psiG) (at gas
gas is
2
2
G,
2
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